FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051194
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTACACATCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT41270.3926011754252783No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40250.3828979236943894No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39360.3744313609095942No Hit
GTCTATGGTGGGTCCTTCAGTGGTTACTTCTGGAGCTGGATCCGCCAGCC39180.37271902236884913No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31820.3027034020361608No Hit
GTGTAACCCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGTGTGAAATCTGTC31710.3016569729279277No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27480.26141701722041794No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27410.26075110778790594No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24190.2301192738923548No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCA23430.22288940005365326No Hit
GATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCACGGTTCCGGG23100.21975011272895392No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22760.2165156954853243No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22760.2165156954853243No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22710.21604004589067288No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC22200.21118842002522845No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21950.2088101720519714No Hit
ATAATATACAGCCGTGTCCGCGGCGGTCACAGAGGTTAGTTTCAGGGAGA21910.20842965237625025No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCA21670.20614653432192345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21540.20490984537582974No Hit
GTCCTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCACGGTT20970.19948743999680363No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20940.19920205024001278No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20660.19653841250996487No Hit
GCATAGATCAGGACCTTAGGGGTTCTCCCTGGCTTCTGCTGATACCAATT20180.19197217640131126No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGACCACTAGTA19360.18417152304902806No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18970.18046145621074702No Hit
CCTTAGGGGTTCTCCCTGGCTTCTGCTGATACCAATTTACATAAGTACTA18950.18027119637288647No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18900.17979554677823503No Hit
GATCAGGACCTTAGGGGTTCTCCCTGGCTTCTGCTGATACCAATTTACAT18730.17817833815642023No Hit
GTCGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGACCACTAGTACTTATGTAAATTGGTATCAGCAGAAG17920.1704728147230673No Hit
GTGTGAAATCTGTCCCAGATCCACTGCCCCGGAACCGTGATGGGACCCCA17780.16914099585804335No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17490.16638222820906512No Hit
GATTTCACACTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGGGTTACACATCC17440.1659065786144137No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAAGTGCCAGATGTGACAT17400.16552605893869257No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16990.16162573226255098No Hit
GTTCCGGGGCAGTGGATCTGGGACAGATTTCACACTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA16920.16095982283003898No Hit
CTGCTACTCTGGCTCCGAAGTGCCAGATGTGACATCCAGATGACCCAGTC16620.1581059252621305No Hit
GGCCAAAAGTGTACGGGGATGTGTAACCCTGTTGACAGAAGTAAGTTGCA15550.1479270239365902No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15530.14773676409872963No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15490.14735624442300851No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14940.14212409888184294No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGA14310.1361309139892351No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAAGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14250.1355601344756534No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCC14100.13413318569169913No Hit
GTATATTATTGTGCGAGAAGGACGGAAATGGGTCTAAGGTTCAACTACGC14080.13394292585383857No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14070.1338477959349083No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13780.1310890282859301No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT13540.12880591023160332No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13530.12871078031267302No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13450.12794974096123074No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13390.12737896144764904No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12950.1231932450147166No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12920.12290785525792575No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12570.11957830809536585No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12250.11653415068959677No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAG12030.11444129247313055No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11990.11406077279740942No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11920.11339486336489746No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11660.11092148547271008No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11640.11073122563484952No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11620.11054096579698894No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11600.11035070595912838No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11580.11016044612126782No Hit
CCATTACTTGCCGGGCAAGTCAGACCACTAGTACTTATGTAAATTGGTAT11570.11006531620233752No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCAGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC11200.10654550920191706No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11190.10645037928298678No Hit
CAATAATATACAGCCGTGTCCGCGGCGGTCACAGAGGTTAGTTTCAGGGAGAATTGGTTCTTGGACGTGTCTA11160.10616498952619592No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11130.10587959976940509No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11030.10492830058010225No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAAGAGTCCTGCGCCCCACTGCTGTACCTGCAC10900.10369161163400857No Hit
TCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTC10880.10350135179614799No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10750.10226466285005431No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10740.10216953293112403No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAAC10720.10197927309326348No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10670.10150362349861206No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10630.10112310382289091No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10620.10102797390396064No Hit
GCCCCGGAACCGTGATGGGACCCCACTTTGCAAATTTGATGCAGCATAGATCAGGACCTTAGGGGTTCTCCCTGG10620.10102797390396064No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10560.10045719439037895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3650.050.2032541
GACTCGA3900.048.33332426
ACTCGAC3950.046.86527
ACGAGAC4100.046.78701822
ATCTCGT3350.046.26484737
GACGTCA4050.045.7797831
TCGTATG3850.045.750940
TCGACGT4050.045.74051729
CTCGACG4050.045.7230828
ATGCCGT3950.045.6487944
ACGTCAT3900.044.91439432
GCCGTCT3950.044.8638546
CGAGACT4250.044.3339423
ATCTCCG4350.044.08342710
TATACAC4450.043.86233
AGACTCG4300.043.83094425
CATCTCG3550.043.63958736
CGACGTC4350.043.395330
CACGAGA4350.043.30858221
GTGGTAT2850.043.2794951
Produced by FastQC (version 0.11.5)