FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051194
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTACACATCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT41370.3935524746145811No Hit
GTCTATGGTGGGTCCTTCAGTGGTTACTTCTGGAGCTGGATCCGCCAGCC38880.36986512480094064No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38790.3690089555305681No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32210.3064134688744418No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32080.30517677992834813No Hit
GTGTAACCCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGTGTGAAATCTGTC31660.3011813233332763No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31550.30013489422504314No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26020.24752804905659656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25830.2457205805969212No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23700.2254579078647709No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCA22540.21442283726885808No Hit
GTCCTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCACGGTT21930.2086199122141108No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21900.20833452245731998No Hit
ATAATATACAGCCGTGTCCGCGGCGGTCACAGAGGTTAGTTTCAGGGAGA21760.20700270359229597No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCA20990.19967769983466419No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20790.19777510145605853No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20610.19606276291531344No Hit
GTGTGAAATCTGTCCCAGATCCACTGCCCCGGAACCGTGATGGGACCCCA20510.19511146372601063No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20230.1924478259959627No Hit
GATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCACGGTTCCGGG19530.18578873167084287No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19240.18302996402186467No Hit
GCATAGATCAGGACCTTAGGGGTTCTCCCTGGCTTCTGCTGATACCAATT18800.1788442475889322No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18710.17798807831855967No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18470.17570496026423288No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18370.17475366107493004No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGACCACTAGTA18290.17399262172348776No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18180.17294619261525465No Hit
GATCAGGACCTTAGGGGTTCTCCCTGGCTTCTGCTGATACCAATTTACAT18110.17228028318274266No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17900.17028255488520674No Hit
CCTTAGGGGTTCTCCCTGGCTTCTGCTGATACCAATTTACATAAGTACTA17560.1670481376415771No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17370.16524066918190172No Hit
GTCGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGACCACTAGTACTTATGTAAATTGGTATCAGCAGAAG17210.1637185904790172No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAAGTGCCAGATGTGACAT16450.15648871664031566No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16290.15496663793743115No Hit
GATTTCACACTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGGGTTACACATCC16260.1546812481806403No Hit
CTGCTACTCTGGCTCCGAAGTGCCAGATGTGACATCCAGATGACCCAGTC16200.1541104686670586No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15960.1518273506127318No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15900.1512565710991501No Hit
GTTCCGGGGCAGTGGATCTGGGACAGATTTCACACTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA15090.14355104766579718No Hit
GGCCAAAAGTGTACGGGGATGTGTAACCCTGTTGACAGAAGTAAGTTGCA14630.13917507139500415No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14100.13413318569169913No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14030.13346727625918717No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCC13470.1281400007990913No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAAGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13430.12775948112337018No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGA13360.12709357169085822No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13110.12471532371760112No Hit
GTATATTATTGTGCGAGAAGGACGGAAATGGGTCTAAGGTTCAACTACGC12910.12281272533899547No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12740.12119551671718065No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12690.12071986712252925No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12540.11929291833857499No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12310.11710493020317847No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11810.11234843425666434No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11790.11215817441880375No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11780.11206304449987348No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11760.11187278466201292No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11760.11187278466201292No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11690.11120687522950094No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11650.1108263555537798No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11340.10787732806694102No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11320.10768706822908046No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAG11070.10530882025582339No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10740.10216953293112403No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCAGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC10670.10150362349861206No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10650.10131336366075148No Hit
CTGCTGATGGTGAGTGTGAAATCTGTCCCAGATCCACTGCCCCGGAACCG10650.10131336366075148No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10560.10045719439037895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGATTG12750.044.0968570
GTGGTAT2950.043.988781
GTATCAA24700.036.361281
GAATACG1201.8189894E-1234.1199469
ATACGTA1201.8189894E-1234.11994611
TACGTAC1201.8189894E-1234.11994612
GTGGATT12050.032.4826469
TATCAAC27650.032.085382
CGGGACT652.4876685E-531.52532817
GTGCTAG1307.2759576E-1231.5223271
ACGTACC1307.2759576E-1231.49833313
ACGGGAC1208.731149E-1131.2974616
ATCAACG28450.031.0602663
TCAACGC28650.030.9644
CAACGCA28950.030.6431275
AACGCAG29450.030.354596
CGTACCA1351.0913936E-1130.33605414
AGGTACG450.00487873230.3302822
AATACGT1351.0913936E-1130.32883810
ACGCAGA30450.029.4697747
Produced by FastQC (version 0.11.5)