FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694990
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGGGTTCATCCATCCCATCCACTCAAGCCCTTGTCCAGTGGCCTG18450.26547144563231123No Hit
CCCTAATAGTGGTGACACAGGCTATGCACAGAAGTTCCAGGGCAGAGTCA17440.2509388624296752No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17410.25050720154246825No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16040.2307946876933481No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGACACACCTTCACCA13540.19482294709276393No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12780.18388753795018634No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12040.1732399027324134No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11550.16618944157469892No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11080.1594267543417891No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11030.1587073195297774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10870.15640512813134003No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10730.15439071065770732No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10700.15395904977050032No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10630.15295184103368395No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10610.15266406710887928No Hit
CAATAATATATGGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCAAATA10480.15079353659764888No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10350.1489230060864185No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10160.1461891538007741No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9880.1421603188535087No Hit
GTTCTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGGCACAGATT9680.13928257960546195No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9670.1391386926430596No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG9160.13180045756054046No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC9130.13136879667333343No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT9090.13079324882372406No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8690.1250377703276306No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8680.12489388336522829No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8540.12287946589159557No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8470.12187225715477921No Hit
CCTCCATAGGAACAGCCTATTTGGAGCTGAGCAGCCTGAGATCTGAAGAC8430.12129670930516986No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8300.11942617879393948No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA8150.11726787435790444No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7980.1148217959970647No Hit
ATATTATTGTGCGAGAGGCCGGTCTCATGACTGTGATTCTAGTAATTGTC7910.11381458726024835No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7910.11381458726024835No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7860.11309515244823666No Hit
ATAATATATGGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCAAATAGGCTGTTCCTATGGAGGTGTCCCTGGT7800.11223183067382264No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT7740.11136850889940864No Hit
GCTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG7640.10992963927538525No Hit
CTCCCAGGTGCAACTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCGGTGAAGGTCTCCTGCAAGG7550.10863465661376422No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT7520.10820299572655721No Hit
GGCTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACCTC7480.10762744787694786No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGATGACCGGGGTTCCCTGG7450.10719578698974086No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7320.10532525647851047No Hit
GCCATATATTATTGTGCGAGAGGCCGGTCTCATGACTGTGATTCTAGTAATTGTCCCCCTGGTGACCACTGGGGC7300.10503748255370582No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7160.10302306508007311No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7100.10215974330565908No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7100.10215974330565908No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA7090.10201585634325673No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA7000.10072087368163572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTCG200.00722352251.9915743
TTAATAC306.6853693E-445.4632723
CGTATGC1700.042.70726841
TTAACGG651.1872544E-842.2549135
TCGTATG1650.041.86641340
CGTACTA1750.041.02615729
ACCGTAC1750.040.97886327
GGTATCA6350.040.2783741
ACGAGAC1800.039.79467822
GTACTAG1900.039.60950530
TAACGGC702.2671884E-839.26799436
GTTTAAC702.318302E-839.16860233
GTATGCC2000.038.06590342
AATGGTA2500.037.93387270
GACCGTA1900.037.72194326
TATACAC1900.037.686663
GTATCAA15950.036.988771
ACTAGAT1900.036.05548532
CTAGATC1950.035.1513133
CACGAGA2050.034.94166621
Produced by FastQC (version 0.11.5)