FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694990
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGGGTTCATCCATCCCATCCACTCAAGCCCTTGTCCAGTGGCCTG17680.25439214952733136No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16680.24000345328709766No Hit
CCCTAATAGTGGTGACACAGGCTATGCACAGAAGTTCCAGGGCAGAGTCA15980.2299313659189341No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15060.21669376537791912No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14970.2153987827162981No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGACACACCTTCACCA12880.1853264075742097No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11960.1720888070331947No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11660.16777219816112462No Hit
CAATAATATATGGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCAAATA11560.16633332853710125No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10870.15640512813134003No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10380.14935466697362554No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10090.14518194506395776No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10030.14431862328954373No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA9500.13669261428221988No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC9360.13467819680858717No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9200.13237600541014977No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8600.12374278766600957No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8560.12316723981640022No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8370.12043338753075583No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT8250.11870674398192779No Hit
CCTCCATAGGAACAGCCTATTTGGAGCTGAGCAGCCTGAGATCTGAAGAC8240.11856285701952547No Hit
ATATTATTGTGCGAGAGGCCGGTCTCATGACTGTGATTCTAGTAATTGTC8240.11856285701952547No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8230.11841897005712312No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8210.11813119613231844No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8120.11683621347069742No Hit
GTTCTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGGCACAGATT8060.1159728916962834No Hit
ATAATATATGGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCAAATAGGCTGTTCCTATGGAGGTGTCCCTGGT7920.1139584742226507No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7920.1139584742226507No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG7870.113239039410639No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7840.11280737852343199No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7810.11237571763622499No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7700.11079296104979927No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7700.11079296104979927No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7680.1105051871249946No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7590.10921020446337357No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7520.10820299572655721No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7450.10719578698974086No Hit
GTACGGGACTCAACAACCACATCTGTCCTCTAGAGAAAACCCTGTGAGCACAGCTCCTCACCATGGACTGGAC7090.10201585634325673No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGATGACCGGGGTTCCCTGG7080.1018719693808544No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAG7030.10115253456884273No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6990.10057698671923337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTG200.00625010853.9294763
GTATCAA14850.040.3588941
ACTAAGG350.001253959740.00934650
GGTATCA5500.039.0059551
AAAGTGG7550.033.59359770
TATCAAC18200.032.5302242
TCAACGC18550.031.7353024
TAACGGC652.3810248E-531.7257736
GTGGTAT2050.031.5611461
TGGTATC2050.031.5361672
GACAGGT1307.2759576E-1231.4333691
CAACGCA19000.031.1672575
AACGCAG19150.030.754266
TTAACGG703.9672013E-529.44038835
AGAGTAC20000.029.2769911
CGCAGAG20150.029.0590468
CAGAGTA20300.028.50892410
CAGGATT605.84992E-428.3773481
ACGGGTT756.7257424E-527.24617616
CGTACCA500.00824396227.24029214
Produced by FastQC (version 0.11.5)