FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927906
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACAGTAGTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT63030.6792713917142469No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35950.3874314855168519No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35590.3835517821848334No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCAGAGAGTTCAGCCTCAGGGAGA34270.3693262033007654No Hit
CTCCTAATCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCC28380.30584994600746196No Hit
CTGTAATACTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC27440.29571960952941356No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24340.26231105305925384No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22950.24733108741618226No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22880.24657670065717863No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22740.24506792713917142No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22110.238278446308139No Hit
GATTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTAGAAAACACT21520.23192004362510857No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21210.22857918797809262No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20260.21834108196304367No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20190.2175866952040401No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19580.21101275344700865No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18940.2041155030789757No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18700.20152903419096332No Hit
GTTTATTACTGTGCGAGACAGAACAGCAACTGGTGGAACTGGTTCGACCC18570.20012803020995662No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18440.19872702622894994No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17530.18891999836190304No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17000.18320821290087574No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16860.18169943938286853No Hit
GTTTTCTACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA16580.17868189234685408No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16550.17835858373585256No Hit
TTATTACTGTCAGCAGTATTACAGTAGTCCTCCGACGTTCGGCCAAGGGA15820.17049140753481495No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC15670.16887486447980724No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCATGTCCAGCCAGAGTGTTTTCTA15210.16391746577778352No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15160.16337861809278095No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14910.16068437966776808No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14670.1580979107797557No Hit
CTTTATTACTGTCAGCAGTATTACAGTAGTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13990.15076958226372067No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13740.1480753438387078No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13690.14753649615370523No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13350.1438723318956877No Hit
AACCAGGACAGCCTCCTAATCTGCTCATTTACTGGGCATCTACCCGGGAA13340.1437645623586872No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCATGTCCAGCCAGAGTGTTTTCTACAGCTCCAA12900.13902270273066453No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12790.13783723782365886No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12780.13772946828665833No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12510.13481969078764444No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGACTA12310.13266430004763413No Hit
GTCTCTGGTGGCTCCATCGGCTGTAGTGGTTGCTACTGGGGCTGGATCCG12140.13083221791862537No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCACTTTATTACTGTCAGCAGTATTACAGTAGT12140.13083221791862537No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11830.1274913622716094No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT11650.12555151060560016No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11570.12468935430959602No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCATGTCCAGC11410.12296504171758776No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11350.12231842449558468No Hit
GTCCCCGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC11270.12145626819958055No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11270.12145626819958055No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10950.11800764301556407No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10940.11789987347856357No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC10840.11682217810855841No Hit
CTATTATGCTGGGAGCACCCACTACAACCCGTCCCTCCAGAGTCGAGTCACCATATCCGTCGACACGTCGAAGAA10820.1166066390345574No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT10530.11348132246154245No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10520.11337355292454193No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10490.11305024431354038No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10380.1118647794065347No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCATGTCCAGCCAGAGTGTTTTCTACAGCTCCAACAATAAGAACTA10170.1096016191295239No Hit
CCCTGAGGAGACGGTGACCACGGTTCCCTGGCCCCAGGGGTCGAACCAGT10040.1082006151485172No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT10020.10798507607451618No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9860.10626076348250793No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9850.10615299394550741No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9780.1053986071865038No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCCGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT9770.10529083764950328No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9720.10475198996450072No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9610.10356652505749504No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT9410.10141113431748475No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9330.10054897802148062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2250.055.3035441
TCGTATG2200.054.9293540
GACGGAT9100.052.76469470
TAAAACG200.006936139452.5294951
GAATGAC200.0073042451.84706541
ATCTCGT2300.050.88456737
ATGCCGT2500.049.96957444
CTTATAC2500.049.1507531
CGAGACG2550.048.194823
CACGAGA2550.048.1870121
GGTATCA7000.047.785451
GTATGCC2650.046.99174542
TACATCT2550.045.75411634
GCCGTCT2750.045.52667646
TTATACA2700.045.495242
CCGTCTT2700.045.13873347
AGACGGA2750.044.7090125
TGCCGTC2800.044.6597445
TGCTTGA2950.044.2090255
TCCGAGC2800.043.8704113
Produced by FastQC (version 0.11.5)