FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927906
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACAGTAGTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT59600.6423064405230703No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36930.39799290014290234No Hit
CTGTAATACTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC34650.373421445706785No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32880.3543462376576938No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCAGAGAGTTCAGCCTCAGGGAGA32840.3539151595096917No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27560.2970128439734197No Hit
CTCCTAATCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCC24720.2664062954652734No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23880.2573536543572302No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23390.2520729470442049No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22930.24711554834218122No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21030.22663933631208336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19830.2137069918720215No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19490.21004282761400403No Hit
GATTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTAGAAAACACT19310.2081029759479947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18910.20379219446797414No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18310.1973260222479432No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18300.1972182527109427No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17900.19290747123092208No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17310.18654906854789172No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16490.17771196651384946No Hit
GTTTATTACTGTGCGAGACAGAACAGCAACTGGTGGAACTGGTTCGACCC16390.1766342711438443No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16270.17534103669983814No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16090.17340118503382887No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15990.17232348966382371No Hit
TTATTACTGTCAGCAGTATTACAGTAGTCCTCCGACGTTCGGCCAAGGGA14820.15971445383476343No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14690.15831344985375673No Hit
GTTTTCTACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA14500.15626582865074695No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14470.1559425200397454No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14400.1551881332807418No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14390.1550803637437413No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14190.15292497300373098No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14120.15217058624472737No Hit
CTTTATTACTGTCAGCAGTATTACAGTAGTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13880.149584117356715No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC13860.14936857828271397No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13460.14505779680269337No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13280.1431179451366841No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCATGTCCAGCCAGAGTGTTTTCTA12600.1357896166206491No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGACTA12450.13417307356564134No Hit
CAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA11960.1288923662526161No Hit
AACCAGGACAGCCTCCTAATCTGCTCATTTACTGGGCATCTACCCGGGAA11660.12565928014260064No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11650.12555151060560016No Hit
GTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA11510.12404273708759292No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCACTTTATTACTGTCAGCAGTATTACAGTAGT11470.12361165893959086No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11390.12274950264358675No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11260.12134849866258005No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT11100.1196241860705718No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11070.11930087745957027No Hit
GTCTCTGGTGGCTCCATCGGCTGTAGTGGTTGCTACTGGGGCTGGATCCG11030.1188697993115682No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10910.11757656486756202No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10790.11628333042355582No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10610.11434347875754657No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCGGGGACCCCGGATTCCCGGGT10430.1124036270915373No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC10380.1118647794065347No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10380.1118647794065347No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCATGTCCAGCCAGAGTGTTTTCTACAGCTCCAA10340.11143370125853266No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10320.11121816218453162No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10180.10970938866652442No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC10060.10841615422251823No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC9930.10701515024151152No Hit
GTCCCCGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC9810.10572191579750535No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC9750.10507529857550225No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCGGGGACCCCG9750.10507529857550225No Hit
CTATTATGCTGGGAGCACCCACTACAACCCGTCCCTCCAGAGTCGAGTCACCATATCCGTCGACACGTCGAAGAA9740.10496752903850173No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9640.10388983366849658No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9590.10335098598349401No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT9460.10194998200248732No Hit
CCCTGAGGAGACGGTGACCACGGTTCCCTGGCCCCAGGGGTCGAACCAGT9440.1017344429284863No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9440.1017344429284863No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT9420.10151890385448525No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9330.10054897802148062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG200.0068143952.7652253
GACGGAT8900.051.74485870
CGTTATA200.007768731651.0448652
GGTATCA8100.047.084151
GCCTACA452.0461393E-645.370773
GTGGTAT2150.044.34671
TGGTATC2300.041.427712
AGCCTAC603.2404023E-739.701562
GTATCAA20100.039.1343081
CGGTAGG350.001411026839.06183629
TCCGAGA5500.038.83250470
CGACGGA8900.035.14543569
ACGGTCG400.002666958634.31096634
TATTCTA1002.2609258E-433.37168570
TATCAAC23550.033.2351532
GACAGGT1550.032.953481
ATCAACG24400.031.9361863
GTGAGCG4600.031.9207470
TCAACGC24450.031.869164
AACGCAG24600.031.8182966
Produced by FastQC (version 0.11.5)