FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892145
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30720.3443386445028555No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAACTCAGGCC27940.31317779060578715No Hit
GTTACAATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAA22390.250968172214158No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20810.23325804661798252No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20030.2245150732224022No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG19720.22104030174467157No Hit
GATCTATGGTGCATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGTG19030.2133061329716582No Hit
ACAATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGA19000.21296986476413587No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA18400.20624450061368949No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17900.2006400304883175No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17820.19974331526825795No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17630.19761361662061658No Hit
GAGTTACAATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCAT16850.1888706432250363No Hit
GTATTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGTGTGAAAT16570.18573213995482798No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGTTCGCCCGTCACAAA15800.1771012559617551No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15800.1771012559617551No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15620.17508364671662116No Hit
CCATAGATCAGGACGTAAGGGGCTTTCCCTGGTTTCTGTTGATACCAATT15350.1720572328489203No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15280.1712726070313682No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT14890.16690112033357807No Hit
GAGTATTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGTGTGAAAT14060.15759769992546055No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13950.15636471649787873No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAA13740.15401083904522248No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13020.1459404020646868No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12970.1453799550521496No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12740.14280189879447847No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12740.14280189879447847No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11950.1339468359963907No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAACTTTTTAAATTGGTATCAACAGAAA11810.13237758436128658No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11740.13159295854373448No Hit
CTGCTACTCTGGCTCCGAGGCGCCCGATGTGACATCCAGATGACCCAGTC11450.12834236587101872No Hit
GTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGTG11290.12654893543089968No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11180.12531595200331785No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10960.12284998514815416No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTATTGTAACTCTGTTGACA10710.12004775008546817No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10580.11859058785287145No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAACAG10440.11702133621776728No Hit
GTGTATTACTGTGCGAGAGGGGGAGACATTGAACGAAGCTGGTTCGACCC10390.11646088920523008No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10380.11634879980272265No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT10290.11533999518015568No Hit
GATTTCACACTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAACAGAGTTACAATACT10280.11522790577764826No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCC10240.11477954816761848No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGCGCCCGATGTGACAT10090.11309820713000689No Hit
GTCTCTGGTGCCTCCATCAGTAATTCCTACTGGAGCTGGATCCGGCAGTC10060.11276193892248458No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9760.10939925684726139No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9600.10760582640714234No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9520.10670911118708282No Hit
GACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT9470.10614866417454562No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAACAGAGTTACAATACTCCGCTCACTTT9260.10379478672188937No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9250.10368269731938194No Hit
GTGTGAAATCTGCCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCA9140.1024497138918001No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9020.10110464106171083No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8960.10043210464666617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTA200.00738745451.69856338
TCGTATG2700.044.79730640
CGTATGC2800.043.2589641
CGAGACA3050.040.1925223
ATGCCGT3100.039.22690644
CACGAGA3000.038.58793621
GGCAATC2800.036.70250333
GTATGCC3500.036.63066542
ACGAGAC3200.036.17618622
CTTATAC3400.036.048991
CCCACGA3250.035.6136419
TATACAC3600.034.9704253
GCAATCT2950.034.86972434
ATCTCCG3400.034.02527610
TTATACA3400.034.0252762
ATCTCGT2950.033.8296937
TACACAT3950.033.5945785
CCGTCTT3700.032.99798647
AGGCAAT3300.032.15054332
ATACACA4000.031.4733814
Produced by FastQC (version 0.11.5)