FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892145
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30770.34489909151539266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30080.33716492274237936No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAACTCAGGCC24750.27742127120591387No Hit
GTTACAATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAA20730.23236133139792298No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19420.21767761966944837No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG19000.21296986476413587No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18390.20613241121118206No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA17420.19525973916796036No Hit
ACAATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGA17410.19514764976545293No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16580.1858442293573354No Hit
GATCTATGGTGCATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGTG16460.18449915652724613No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15740.17642871954671047No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15460.17329021627650215No Hit
GTATTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGTGTGAAAT15420.17284185866647236No Hit
GAGTTACAATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCAT14920.16723738854110037No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGTTCGCCCGTCACAAA14900.1670132097360855No Hit
GAGTATTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGTGTGAAAT14210.15927904096307213No Hit
CCATAGATCAGGACGTAAGGGGCTTTCCCTGGTTTCTGTTGATACCAATT14130.15838232574301264No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13620.1526657662151332No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAA13510.15143278278755135No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13410.15031188876247695No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13210.14807010071232815No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13190.14784592190731327No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13160.14750965369979097No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12810.14358652461203056No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12580.14100846835435943No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11950.1339468359963907No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11920.1336105677888684No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11430.12811818706600384No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGTT11360.12733356124845177No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11350.12722147184594432No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAACTTTTTAAATTGGTATCAACAGAAA10960.12284998514815416No Hit
CTGCTACTCTGGCTCCGAGGCGCCCGATGTGACATCCAGATGACCCAGTC10610.11892685606039377No Hit
GTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGTG10580.11859058785287145No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10430.11690924681525985No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTATTGTAACTCTGTTGACA10370.1162367104002152No Hit
GATTTCACACTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAACAGAGTTACAATACT9960.11164104489741018No Hit
GTGTATTACTGTGCGAGAGGGGGAGACATTGAACGAAGCTGGTTCGACCC9910.11108059788487298No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCC9910.11108059788487298No Hit
GTGTGAAATCTGCCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCA9580.10738164760212746No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9560.10715746879711259No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAACAG9520.10670911118708282No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9500.10648493238206794No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGCGCCCGATGTGACAT9450.10592448536953074No Hit
GTCTCTGGTGCCTCCATCAGTAATTCCTACTGGAGCTGGATCCGGCAGTC9430.10570030656451584No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9380.10513985955197866No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9340.1046915019419489No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTATTGTCAACAGAGTTACAATACTCCGCTCACTTT9250.10368269731938194No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9180.10289807150182986No Hit
GACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT9050.10144090926923315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAT6500.044.0340670
GTGGTAT2450.043.008871
GATTTAG750.002524552634.693570
ATCCGCT400.002808492433.9508677
AGCCTAC400.002810036333.9470634
ATCAACG28800.031.3538863
AATGGTA4000.030.898970
TCAACGC29450.030.6618634
AACGCAG29650.030.5729586
CAACGCA29950.030.3783745
CTAGTGA6750.029.1149169
ACGCAGA31300.028.9612837
CGCAGAG31350.028.8067978
AGAGTAC31600.028.36401611
GCAGAGT31800.028.292399
GCTTGTT854.969488E-627.9924891
TATATAC656.5675593E-427.8221964
GTATCAA33300.027.6622491
GCACCGT500.00836373827.1606926
TATAGGA500.00836831227.1576482
Produced by FastQC (version 0.11.5)