FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868509
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT30320.3491040392212401No Hit
GGACTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC30050.34599526314638074No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC29180.33597809579405624No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23110.2660882040370336No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22960.2643611062176673No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC21450.24697498816937993No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18860.21715376582165527No Hit
GACTATTACTGTAGCTCTCGGGACAGCAGTGCTCACCACGGGGTCTTCGGGTCCGGGACCAGGGTCATCGTCCTG17550.20207044486585635No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTACTGTGTCTGTGGCCTTGGGA17530.2018401651566075No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16970.19539233329763997No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTTAGGGAAG16760.19297439635052718No Hit
GGACAGCAGTGCTCACCACGGGGTCTTCGGGTCCGGGACCAGGGTCATCGTCCTGCGTCAGCCCAAGGCCAACCC16590.19101701882191202No Hit
ACCCTACTGTGTCTGTGGCCTTGGGACAGACAGTCACAATCACATGCCAAGGAGACAACCTCAGAAATTACTATG16530.19032617969416551No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGCAGGACGATGACCCTGGTCCCGGACCCGAAGACCCCGTGGTGAG16470.189635340566419No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTACT16130.18572058550918874No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16000.1842237673990713No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15850.18249666957970498No Hit
CTATTATTGTGCGAGGGAGTGGACGTATTACGATGCTTTGACTGGCCCCT15800.18192097030658289No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15760.1814604108880852No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15660.18030901234184102No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15540.17892733408634798No Hit
CTGCTGTCCCGAGAGCTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGC15230.17535799859299098No Hit
GGACCCTACTGTGTCTGTGGCCTTGGGACAGACAGTCACAATCACATGCC14340.16511055153141765No Hit
CTATTACTGTAGCTCTCGGGACAGCAGTGCTCACCACGGGGTCTTCGGGT14330.1649954116767932No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA14250.16407429283979785No Hit
TGCCGGGACAGGCCCCGGTCCTCGTCATCCATGGGAACAACAAGCGGCCC13780.15866271967245016No Hit
GTCCCGGCATCTGCCGGTACCAGCTGGCATAGTAATTTCTGAGGTTGTCTCCTTGGCATGTGATTGTGACTGT13520.15566908345221522No Hit
GTCTTCGGGTCCGGGACCAGGGTCATCGTCCTGCGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCC13360.15382684577822453No Hit
GTCACAATCACATGCCAAGGAGACAACCTCAGAAATTACTATGCCAGCTGGTACCGGCAGATGCCGGGACAGGCC13160.15152404868573613No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12850.14795471319237913No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12640.1455367762452663No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12400.14277341973428023No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11920.1372467067123081No Hit
GTCTGTGGCCTTGGGACAGACAGTCACAATCACATGCCAAGGAGACAACCTCAGAAATTACTATGCCAGCTGGTA11830.13621044802068832No Hit
CAATAATAGAGGGCTGTGTCCTCGGGTCCCAGGTTGTTCATTTGAAGATA11810.13598016831143947No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11750.135289329183693No Hit
GCCTTGGGCTGACGCAGGACGATGACCCTGGTCCCGGACCCGAAGACCCCGTGGTGAGCACTGCTGTCCCGAGAG11710.1348287697651953No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGGACTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACT11370.13091401470796502No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11310.1302231755802185No Hit
CCCGAAGACCCCGTGGTGAGCACTGCTGTCCCGAGAGCTACAGTAATAGT11090.12769009877848128No Hit
CTCTAAAAGGTGTCCAGTGTGAAGTGCAGTTGGTGGAGTCTGGGGGAGGC10990.12653870023223707No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTTAGGGA10940.12596300095911497No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTACTGTGTCTGTGG10930.12584786110449056No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10650.12262394517500681No Hit
CCACGGGGTCTTCGGGTCCGGGACCAGGGTCATCGTCCTGCGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT10530.12124226691951377No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10400.11974544880939633No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10310.11870919011777656No Hit
CTACTGTGTCTGTGGCCTTGGGACAGACAGTCACAATCACATGCCAAGGA10250.11801835099003004No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10170.11709723215303468No Hit
GTGATGGTTAGGGAAGCTGTGTTTCCTGAGGTGGAGCCAGAGAATCGGTC10160.11698209229841025No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10020.1153701343336684No Hit
GCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCACCTCAGGAAACACAG9840.11329761695042884No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9840.11329761695042884No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG9750.11226135825880906No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9740.11214621840418464No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC9710.11180079884031138No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9690.11157051913106254No Hit
GCAGAGGACTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTT9660.11122509956718928No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9510.109498001747823No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9280.10684978509146134No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT9250.10650436552758807No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9190.10581352639984158No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9160.10546810683596831No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9090.10466212785359738No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT9080.10454698799897295No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC9050.1042015684350997No Hit
GGGTCATCGTCCTGCGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG9000.1036258691619776No Hit
GCCTCATCTTCCGCCTGAGCCCCAGTGATGGTTAGGGAAGCTGTGTTTCCTGAGGTGGAGCCAGAGAATCGGT8940.10293503003423107No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGGTTCACCGTCGCCACCCACTAC8910.10258961047035782No Hit
CTTCTGAGCTGACTCAGGACCCTACTGTGTCTGTGGCCTTGGGACAGACA8910.10258961047035782No Hit
GCCTTGGGACAGACAGTCACAATCACATGCCAAGGAGACAACCTCAGAAA8870.10212905105186014No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG8800.10132307206948919No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC8780.10109279236024038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1500.048.30903640
TCTCGTA1500.045.92849738
GTGGTAT2350.043.6397671
CTTATAC1800.041.7810331
GGTATCA7450.040.837841
AGGGGGG2600.040.4829970
TACATCT1700.040.37517534
TGGTATC2550.040.1985132
CGAGACG1800.039.87730423
ACGAGAC1800.039.87500822
TTATACA1900.039.5638052
ATCTCGT1750.039.32620237
CACGAGA1850.038.79730221
ATCTCCG1850.038.7883710
TCTCCGA1850.038.7883711
TATACAC1950.038.5493473
GTATCAA18700.038.3889921
GCTACAT1800.038.0836632
CCGTCTT1950.037.44647
ATGCCGT1950.037.29088244
Produced by FastQC (version 0.11.5)