FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868509
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC28610.32941512408046436No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28400.3269971871333515No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT27730.31928281687351545No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23160.26666390331015566No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG21780.2507746033719858No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19700.22682551361010653No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18750.21588722742078664No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTACTGTGTCTGTGGCCTTGGGA18180.20932425570719476No Hit
CTGCTGTCCCGAGAGCTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGC17600.20264614413897838No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17180.19781027024475278No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTACT16800.19343495576902486No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16460.1895202007117946No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15960.1837632079805736No Hit
GACTATTACTGTAGCTCTCGGGACAGCAGTGCTCACCACGGGGTCTTCGGGTCCGGGACCAGGGTCATCGTCCTG15870.18272694928895383No Hit
ACCCTACTGTGTCTGTGGCCTTGGGACAGACAGTCACAATCACATGCCAAGGAGACAACCTCAGAAATTACTATG15600.1796181732140945No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTTAGGGAAG15460.17800621524935262No Hit
CTATTATTGTGCGAGGGAGTGGACGTATTACGATGCTTTGACTGGCCCCT15440.1777759355401038No Hit
GGACAGCAGTGCTCACCACGGGGTCTTCGGGTCCGGGACCAGGGTCATCGTCCTGCGTCAGCCCAAGGCCAACCC15290.17604883772073748No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15230.17535799859299098No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15210.17512771888374215No Hit
GGACCCTACTGTGTCTGTGGCCTTGGGACAGACAGTCACAATCACATGCC15000.17270978193662934No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGCAGGACGATGACCCTGGTCCCGGACCCGAAGACCCCGTGGTGAG14900.17155838339038512No Hit
CTATTACTGTAGCTCTCGGGACAGCAGTGCTCACCACGGGGTCTTCGGGT14150.16292289429355367No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13790.15877785952707457No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13490.15532366388834198No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA13450.1548631044698443No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13450.1548631044698443No Hit
GTCCCGGCATCTGCCGGTACCAGCTGGCATAGTAATTTCTGAGGTTGTCTCCTTGGCATGTGATTGTGACTGT12820.14760929362850586No Hit
GTCTTCGGGTCCGGGACCAGGGTCATCGTCCTGCGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCC12700.14622761537301282No Hit
GTCACAATCACATGCCAAGGAGACAACCTCAGAAATTACTATGCCAGCTGGTACCGGCAGATGCCGGGACAGGCC12480.1436945385712756No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12120.1395495038047965No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11980.13793754584005463No Hit
TGCCGGGACAGGCCCCGGTCCTCGTCATCCATGGGAACAACAAGCGGCCC11840.13632558787531274No Hit
CAATAATAGAGGGCTGTGTCCTCGGGTCCCAGGTTGTTCATTTGAAGATA11300.1301080357255941No Hit
GCCTTGGGCTGACGCAGGACGATGACCCTGGTCCCGGACCCGAAGACCCCGTGGTGAGCACTGCTGTCCCGAGAG11280.12987775601634527No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11090.12769009877848128No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11070.12745981906923243No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10780.12412076328512428No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGGACTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACT10770.12400562343049985No Hit
GTCTGTGGCCTTGGGACAGACAGTCACAATCACATGCCAAGGAGACAACCTCAGAAATTACTATGCCAGCTGGTA10530.12124226691951377No Hit
CCCGAAGACCCCGTGGTGAGCACTGCTGTCCCGAGAGCTACAGTAATAGT10030.11548527418829282No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9800.11283705753193116No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGCAGGACGATGACCCTGGTCCCGG9690.11157051913106254No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9670.1113402394218137No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9620.11076454014869162No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9620.11076454014869162No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9570.11018884087556952No Hit
CCACGGGGTCTTCGGGTCCGGGACCAGGGTCATCGTCCTGCGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT9460.1089223024747009No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9430.10857688291082764No Hit
GCCTTGGGACAGACAGTCACAATCACATGCCAAGGAGACAACCTCAGAAA9390.10811632349232995No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9390.10811632349232995No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTTAGGGA9340.10754062421920786No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9300.10708006480071018No Hit
CTCTAAAAGGTGTCCAGTGTGAAGTGCAGTTGGTGGAGTCTGGGGGAGGC9280.10684978509146134No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTACTGTGTCTGTGG9270.10673464523683691No Hit
GCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCACCTCAGGAAACACAG9270.10673464523683691No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC9270.10673464523683691No Hit
AACCATCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAGCT9240.10638922567296366No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC9180.10569838654521715No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG8990.10351072930735318No Hit
CTTCTGAGCTGACTCAGGACCCTACTGTGTCTGTGGCCTTGGGACAGACA8890.10235933076110898No Hit
GCAGAGGACTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTT8880.10224419090648455No Hit
GTGATGGTTAGGGAAGCTGTGTTTCCTGAGGTGGAGCCAGAGAATCGGTC8880.10224419090648455No Hit
CTACTGTGTCTGTGGCCTTGGGACAGACAGTCACAATCACATGCCAAGGA8850.1018987713426113No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT8850.1018987713426113No Hit
GTATCAACGCAGAGTACGGGAGCTGTGGGCTCAGAAGCAGAGGACTGGGGTGTCTCCACCATGGCCTGGACCCCT8730.10051709308711827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)