FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953484
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT35860.37609440745728295No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC32940.34546987678870333No Hit
CTATAGGAGAGGAAGCAGTAATAATTAGACTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC30350.31830633759979193No Hit
CTCCTATAGTGGTGTGGTGGTTTTCGGCGGAGGGACCAAACTGACCGTCC27070.2839061798624833No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC27000.28317203015467485No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAC24620.2582109400891887No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA24140.2531767706642167No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22900.24017183298303907No Hit
GTCCAGGAGTGTTTGATGTTTACATCATAAATCAGTGTCCTGGGGGCTTG22570.2367108415033708No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC21630.22685225971280062No Hit
GCCTGAGGATGAGTCTAATTATTACTGCTTCCTCTCCTATAGTGGTGTGGTGGTTTTCGGCGGAGGGACCAAA20790.21804246321909965No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCACCACACCACTA20600.2160497711550482No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT20130.21112048025976313No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18640.1954935793364126No Hit
GGGTGCGACAGGCCCCTGGAGAAGGACTTGAGTGGATGGGACGGATCCACCTTAACAGTGGTGACACAATCTATG18090.18972526020363215No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17150.179866678413062No Hit
GTCTGGGGCTGAGGTGAAGGCGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGACTTCTGGATACACCTTCGCCGG17050.1788178931161928No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17020.1785032575271321No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC16840.17661544399276757No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16350.17147639603810869No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15970.16749101191000584No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15250.15993975777254785No Hit
CCTTAACAGTGGTGACACAATCTATGCACAGAAGTTTCAGGGTAGGGTCACTGTGACCAGGGACACGTCCATCAA15060.15794706570849643No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGC15040.15773730864912258No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAAGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC14920.15647876629287957No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC14810.1553251024663235No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG14450.1515494753975945No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14430.15133971833822066No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14250.14945190480385617No Hit
GTAATAAACGGCCGTGTCGTCAGAGGTCAGCCTGCTCAGGTCCATGTAGGCCGTGTTGATGGACGTGTCCCTGGT14230.14924214774448233No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14100.14787872685855244No Hit
GGCCTGGCCAAGCCCCCAGGACACTGATTTATGATGTAAACATCAAACAC13740.14410309978982344No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13680.14347382861170194No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13530.14190065066639818No Hit
CCACTATAGGAGAGGAAGCAGTAATAATTAGACTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG13530.14190065066639818No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13150.13791526653829533No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13050.1368664812414262No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA12810.13434939652894018No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12570.1318323118164542No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12560.13172743328676725No Hit
GATTTATGATGTAAACATCAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC12500.13109816210864575No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12320.12921034857428126No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12250.12847619886647285No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12250.12847619886647285No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12230.128266441807099No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC12140.12732253503991675No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12130.12721765651022987No Hit
GCACTGGAGCTGTCACCAGTGGTCATTTTCCCTACTGGTTCCAGCAGAGG12020.12606399268367377No Hit
CCTATAGTGGTGTGGTGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTA11810.12386154356024853No Hit
GTGGTGACTCAAGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC11760.12333715091181394No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11750.12323227238212701No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11700.12270787973369243No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11530.12092494472901487No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAG11310.11861761707590268No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCG11260.1180932244274681No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAAC11140.1168346820712251No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11120.11662492501185127No Hit
ACATGGACCTGAGCAGGCTGACCTCTGACGACACGGCCGTTTATTACTGTGCCAGCCTCGGTGGTACGTTCGAGT10990.11526150412592136No Hit
GTTTATTACTGTGCCAGCCTCGGTGGTACGTTCGAGTCCCGGTCCGACCC10820.11347856912124377No Hit
GGTCCATGTAGGCCGTGTTGATGGACGTGTCCCTGGTCACAGTGACCCTA10800.11326881206186995No Hit
TAATTAGACTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAAC10780.1130590550024961No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10690.11211514823531386No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC10580.11096148440875778No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAG10320.10823464263689794No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10240.10739561439940262No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10220.10718585734002878No Hit
CTGTTAAGGTGGATCCGTCCCATCCACTCAAGTCCTTCTCCAGGGGCCTG9940.1042492585087951No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC9900.10382974439004744No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9810.10288583762286521No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9630.1009980240885007No Hit
CCCTTTCGGGTGCGCAGCCTGAGGATGAGTCTAATTATTACTGCTTCCTC9590.10057850996975304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4800.089.0552470
TCGTATG5800.051.84012240
CGTATGC5950.051.78061341
GTCGTAT200.00735764751.75171739
TTATCGG200.00784033150.9266479
ACGAGAC5900.050.08186722
TGGTATC2200.049.3808252
TATCTCG5600.049.0416736
TCTCGTA5700.048.96010638
ATCTCCG6200.048.7362510
TCTCCGA6150.048.5804311
CTTATAC6300.048.529561
ATGCCGT6400.048.33622444
GACCGGA6100.047.92328326
CACGAGA6100.047.87552321
CCGTCTT6500.047.79531547
TTATACA6400.047.7412262
GCCTATC5750.047.57563433
ACCGGAG6150.047.55863627
TATACAC6500.047.529043
Produced by FastQC (version 0.11.5)