FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953484
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60160.6309492345964903No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC32950.3455747553183902No Hit
CTATAGGAGAGGAAGCAGTAATAATTAGACTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC27440.2877866854608992No Hit
CTCCTATAGTGGTGTGGTGGTTTTCGGCGGAGGGACCAAACTGACCGTCC26800.2810744595609365No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC25710.2696426998250626No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA24800.2600987536235532No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAC23390.24531088093769798No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23270.24405233858145495No Hit
GTCCAGGAGTGTTTGATGTTTACATCATAAATCAGTGTCCTGGGGGCTTG21810.2287400732471651No Hit
GCCTGAGGATGAGTCTAATTATTACTGCTTCCTCTCCTATAGTGGTGTGGTGGTTTTCGGCGGAGGGACCAAA21250.22286687558469778No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT20330.21321805085350148No Hit
GGGTGCGACAGGCCCCTGGAGAAGGACTTGAGTGGATGGGACGGATCCACCTTAACAGTGGTGACACAATCTATG19780.2074497317207211No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC19600.20556191818635658No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCACCACACCACTA17650.18511060489740783No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17230.18070570665055732No Hit
GTCTGGGGCTGAGGTGAAGGCGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGACTTCTGGATACACCTTCGCCGG16540.17346908810216008No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16190.16979833956311802No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15960.1673861333803189No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15930.16707149779125816No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC15790.16560319837564133No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15710.16476417013814598No Hit
GTAATAAACGGCCGTGTCGTCAGAGGTCAGCCTGCTCAGGTCCATGTAGGCCGTGTTGATGGACGTGTCCCTGGT14670.15385680305070668No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14590.15301777481321133No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14530.15238850363508985No Hit
CCTTAACAGTGGTGACACAATCTATGCACAGAAGTTTCAGGGTAGGGTCACTGTGACCAGGGACACGTCCATCAA14470.15175923245696832No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAAGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC14460.15165435392728144No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14020.1470396986210571No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAAC13800.14473237096794492No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13660.1432640715523281No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGC13440.14095674389921592No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13340.13990795860234678No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG13270.13917380889453834No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13080.13718111683048692No Hit
GGCCTGGCCAAGCCCCCAGGACACTGATTTATGATGTAAACATCAAACAC12710.13330061123207101No Hit
CCACTATAGGAGAGGAAGCAGTAATAATTAGACTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG12530.13141279769770653No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC12500.13109816210864575No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA12360.12962986269302892No Hit
GATTTATGATGTAAACATCAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC12300.12900059151490742No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12250.12847619886647285No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12130.12721765651022987No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12120.12711277798054293No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11900.12480545032743076No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11880.12459569326805695No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11780.12354690797118777No Hit
GCACTGGAGCTGTCACCAGTGGTCATTTTCCCTACTGGTTCCAGCAGAGG11730.1230225153227532No Hit
CTGTTAAGGTGGATCCGTCCCATCCACTCAAGTCCTTCTCCAGGGGCCTG11540.12102982325870176No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11530.12092494472901487No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11410.11966640237277186No Hit
GGTCCATGTAGGCCGTGTTGATGGACGTGTCCCTGGTCACAGTGACCCTA11380.1193517667837111No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11360.11914200972433728No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11100.11641516795247743No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCG11090.11631028942279054No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10840.11368832618061761No Hit
ACATGGACCTGAGCAGGCTGACCTCTGACGACACGGCCGTTTATTACTGTGCCAGCCTCGGTGGTACGTTCGAGT10780.1130590550024961No Hit
GTGGTGACTCAAGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC10720.11242978382437462No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG10610.11127611999781853No Hit
GTTTATTACTGTGCCAGCCTCGGTGGTACGTTCGAGTCCCGGTCCGACCC10200.10697610028065495No Hit
CCTATAGTGGTGTGGTGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTA10130.10624195057284654No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC10050.1054029223353512No Hit
CCCTTTCGGGTGCGCAGCCTGAGGATGAGTCTAATTATTACTGCTTCCTC9970.10456389409785588No Hit
TAATTAGACTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAAC9960.10445901556816894No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGTCTAATTAT9880.10361998733067362No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAG9850.10330535174161287No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9720.10194193085568296No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9720.10194193085568296No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9690.1016272952666222No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA9580.10047363144006612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)