FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387352
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT32660.8431607426836571No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11710.30230901092546314No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC11570.29869472727648233No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC10850.2801069827960098No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG10300.26590801131787106No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT10180.262810053904459No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC9000.2323468060059068No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC8900.22976517482806336No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC8600.22202028129453313No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8190.21143559346537516No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGGGCACTCA7570.19542948016274603No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGGGCACTC7390.1907825440426279No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7230.18665193415807843No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGATAACAGCAATCGG6780.1750345938577831No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC6760.17451826762221442No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC6700.17296928891550836No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6480.16728970032425286No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA6430.16599888473533117No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT6380.16470806914640948No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG6240.1610937854974287No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6140.15851215431958526No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGGGCA6000.1548978706706045No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG5970.1541233813172515No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT5960.15386521819946714No Hit
GTATTATTGTGCAAGAGATGAGGGAGTAAACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTC5770.14896011896156466No Hit
GATTATTACTGCCAGTCCTATGACATCAGCCTGAGTGCCCTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTC5650.14586216154815257No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG5580.14405501972366222No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG5350.13811726801462235No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG5190.1339866581300729No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5140.13269584254115122No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5100.13166319007001384No Hit
GTCCTATGACATCAGCCTGAGTGCCCTTTATGTCTTCGGAACTGGGACCA5060.13063053759887647No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT5020.12959788512773912No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT4940.12753258018546437No Hit
CAATAATACAAGGCGGTGTCCTCACTTCTCAGACTGTTCATTTGCAGATA4810.12417645965426795No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG4780.12340197030091492No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC4680.12082033912307152No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTACATCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGT4650.12004584976971851No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGATAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG4640.11978768665193416No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTG4550.11746421859187509No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA4500.1161734030029534No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACATCAGCCTGAGTGCCC4470.11539891364960035No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG4370.11281728247175696No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG4360.11255911935397261No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT4350.11230095623618827No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4320.11152646688283524No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4310.11126830376505092No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAAT4290.11075197752948222No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC4290.11075197752948222No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG4240.10946116194056052No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4230.10920299882277618No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC4160.10739585699828579No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT4080.10533055205601106No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA4050.10455606270265805No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC4010.10352341023152067No Hit
GGTCTGGAGTGGGTCTCTCGTGTTACTTGGGATGGTGATAGGACATACTA3990.103007083995952No Hit
ACATCAGCCTGAGTGCCCTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACC3990.103007083995952No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC3960.10223259464259898No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT3960.10223259464259898No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC3960.10223259464259898No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC3890.1004254528181086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)