FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387352
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60801.5696317561287925No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC11530.297662074805345No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT9810.25325801854643837No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC9450.2439641463062021No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9080.23441211094818148No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG8900.22976517482806336No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC8870.22899068547471033No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG8270.2135008984076499No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC7800.20136723187178587No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC7650.19749478510502078No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7410.19129887027819656No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC6670.17219479956215533No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT6580.16987133150209627No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6550.16909684214874326No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG6480.16728970032425286No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGATAACAGCAATCGG6400.16522439538197814No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT6360.16419174291084077No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6260.16161011173299739No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC5920.1528325657283298No Hit
GTATTATTGTGCAAGAGATGAGGGAGTAAACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTC5910.15257440261054545No Hit
GATTATTACTGCCAGTCCTATGACATCAGCCTGAGTGCCCTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTC5710.14741114025485863No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA5590.14431318284144654No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT5500.14198971478138747No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG5430.14018257295689707No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGGGCACTCA5360.13837543113240672No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGGGCACTC5260.13579379995456328No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC5230.13501931060121028No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG5070.1308887007166608No Hit
GTCCTATGACATCAGCCTGAGTGCCCTTTATGTCTTCGGAACTGGGACCA5010.12933972200995478No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT4930.12727441706768006No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG4920.1270162539498957No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTACATCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGT4880.12598360147875834No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC4870.125725438360974No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG4760.12288564406534624No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT4690.12107850224085585No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG4600.11875503418079679No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG4570.11798054482744377No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAAT4560.11772238170965944No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT4530.11694789235630641No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4530.11694789235630641No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA4430.114366261178463No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGGGCA4410.1138499349428943No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4410.1138499349428943No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT4360.11255911935397261No Hit
CAATAATACAAGGCGGTGTCCTCACTTCTCAGACTGTTCATTTGCAGATA4320.11152646688283524No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4230.10920299882277618No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC4190.10817034635163883No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACATCAGCCTGAGTGCCC4140.10687953076271711No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC4110.1061050414093641No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGATAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG4110.1061050414093641No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA4070.10507238893822672No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC3990.103007083995952No Hit
GTCTGAGAAGTGAGGACACCGCCTTGTATTATTGTGCAAGAGATGAGGGA3970.10249075776038333No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT3940.10171626840703031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTGT150.002633809667.07546
TATCCTA200.00605941454.34848458
GTGGTAT1200.047.5670171
CATTCCT350.001045258141.51259659
GGTATCA4450.036.9721151
GTATCAA10200.034.893451
TATCAAC10950.032.7635422
TGGTATC1850.030.8104722
GACAGGT553.8137464E-430.5242921
AACGCAG11700.030.3845846
ATCAACG11850.030.2751713
TCAACGC11850.029.9922264
CATTTGG450.005274161729.8459721
CAACGCA11950.029.7412435
CGCAGAG12300.028.9024098
GTGGGGC500.00640982828.67783454
CAGAGTA12500.028.4399710
ACGCAGA12650.028.1027377
AGAGTAC12700.027.99209811
ATATGTC606.3661154E-427.96613719
Produced by FastQC (version 0.11.5)