FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274898
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53111.931989319674934No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC5450.19825535289452814No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC4920.17897547453964743No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT4850.17642907551164433No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4790.17424644777335593No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG4280.15569411199790467No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4060.14769114362418062No Hit
GTAATAAACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA4050.14732737233446586No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT3810.13859686138131233No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT3790.13786931880188288No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT3660.13314029203559138No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC3450.12550109495158204No Hit
CCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCT3320.12077206818529056No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC3230.11749812657785798No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT3230.11749812657785798No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG3210.1167705839984285No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG3180.11567927012928432No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCAC3120.11349664239099592No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG3100.11276909981156648No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT3090.11240532852185176No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC2950.10731253046584552No Hit
GTTTATTACTGTGCGAGAGGTGAAGAACAGTGGCTGGGCCTAGACTACTGGGGCCGGGGAACCCTGGTCACCGT2910.1058574453069866No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC2900.10549367401727187No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG2850.1036748175686982No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT2840.10331104627898348No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT2780.1011284185406951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACC150.002751298766.335824
GCTTGAC200.003998667560.35886468
CACAAGT200.00852986949.84253724
ATATAGC200.00859148649.7518656
CCATACT200.00859148649.7518654
GACAGGT307.570394E-444.3206331
GTATCAA12000.039.334561
TCGTTAA400.00187669336.8480258
CCGATCC400.00197169136.4793955
TCACGAA450.002828855833.89079365
CGTTCAT400.003001780733.48466530
ATTACCG502.336174E-433.1799815
CGACAGC400.003144952533.167916
CTCGTTA450.003407374532.62850657
AGTGAGT1009.8771125E-932.27608550
TATCAAC14600.032.037852
ACGAATG500.004395162730.97456267
CGTTGTC1101.5381374E-830.84022763
ATCAACG15350.030.6830163
TTGGCGT1101.8155333E-830.31995659
Produced by FastQC (version 0.11.5)