FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617647
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20620.3338476508426334No Hit
GTATTATAATACTCCTCCGGCTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT19760.31992383999274665No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19270.3119905059038577No Hit
CTTTTATACACCTCCAATAATAAGAACTACTTGGCTTGGTACCAGCAGAA15030.24334288031836954No Hit
CTCCTAAGCTGCTCATTTACTGGGGATCTATTCGGGAATCCGGGGTCCCT14610.23654287967075044No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13370.21646668728254165No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13260.21468573473197475No Hit
ATTATAATACTCCTCCGGCTTTCGGCGGAGGGACCAGGGTGGAGATCAAA12140.1965523996716571No Hit
GTATTATAATACTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTACTGATGCTGAGCGTGAAAT11850.19185716112925344No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11810.19120954201995638No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11390.18440954137233725No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10890.17631430250612404No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10810.17501906428752995No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10520.17032382574512628No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10380.16805715886258654No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10310.1669238254213167No Hit
CTCCTGTACCGTCTCCGGACTCACCTTCAGTAACGCCTGGATGACCTGGGTCCGCCAGGCCCCAGGGAAGGGG9890.1601238247736976No Hit
TTATAATACTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTACTGATGCTGAGCGTGAAATCTGTC9850.15947620566440054No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9700.15704763400453656No Hit
GCTTAGGAGGCAGTCCAACTTTCTGCTGGTACCAAGCCAAGTAGTTCTTATTATTGGAGGTGTATAAAAGACT9490.153647633680727No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9460.15316191934875423No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8920.14441906137324392No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8650.14004763238548879No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8620.139561918053516No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT8390.13583810817505793No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8250.13357144129251822No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8210.13292382218322116No Hit
GTTTATTACTGTCAGCAGTATTATAATACTCCTCCGGCTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA7980.12920001230476308No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7820.12660953586757484No Hit
TTATAATACTCCTCCGGCTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT7550.1222381068798197No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7460.12078096388390132No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA7340.11883810655601015No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG7290.11802858266938884No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7050.11414286801360647No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC7000.11333334412698516No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7000.11333334412698516No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACGCTCAG6970.11284762979501235No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGCC6940.11236191546303957No Hit
GTAATAAACCGCCACATCTTCAGCCTGCAGGCTACTGATGCTGAGCGTGAAATCTGTCCCAGACCCGCTGGCA6900.11171429635374253No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6880.11139048679909397No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6830.11058096291247266No Hit
ATTCAGTGCCAGCGGGTCTGGGACAGATTTCACGCTCAGCATCAGTAGCC6830.11058096291247266No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6810.11025715335782414No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC6690.10831429602993295No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6690.10831429602993295No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6670.10799048647528442No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC6590.1066952482566903No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6560.10620953392471752No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG6370.10313334315555649No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6370.10313334315555649No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT6270.10151429538231384No Hit
GTAGTACACGGCTGTGTCCTCAACTTTCAGGCTGCTCATGTGAAGATATA6240.10102858105034106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTC150.002402818868.6512633
TTACCGC200.00765712951.23000316
GGTATCA7800.042.9314581
CGTATGC1300.039.90194741
TCGTATG1300.039.86599740
ATCTCGT1300.039.73906737
ACGCGTA1300.039.47480427
GTATCAA19850.037.6988221
TTACGCG450.001684329237.67592269
CGCGTAG1400.036.66707228
GACGCGT1400.036.65220626
GTAATCT1450.035.532434
AGACGCG1500.034.1976325
GTTACGC450.002720140434.17062868
CTCTTAT807.7099685E-834.1699331
CTAGGAC501.9741576E-434.153343
CTTAGAC400.002727162334.1533363
TGGTATC2350.033.429382
CGAGACG1550.033.0703623
CACGAGA1550.033.0623221
Produced by FastQC (version 0.11.5)