FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617647
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19930.32267622120725914No Hit
GTATTATAATACTCCTCCGGCTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT19360.3134476488997761No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16690.2702190733541975No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15810.25597145294966217No Hit
CTTTTATACACCTCCAATAATAAGAACTACTTGGCTTGGTACCAGCAGAA14920.24156192776780264No Hit
CTCCTAAGCTGCTCATTTACTGGGGATCTATTCGGGAATCCGGGGTCCCT14640.23702859400272325No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13030.21096192485351664No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12800.20723811497505856No Hit
ATTATAATACTCCTCCGGCTTTCGGCGGAGGGACCAGGGTGGAGATCAAA12130.19639049489433286No Hit
GTATTATAATACTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTACTGATGCTGAGCGTGAAAT11900.19266668501587478No Hit
CTCCTGTACCGTCTCCGGACTCACCTTCAGTAACGCCTGGATGACCTGGGTCCGCCAGGCCCCAGGGAAGGGG10950.17728573117006963No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10300.16676192064399242No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9960.16125715821496744No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9810.15882858655510348No Hit
TTATAATACTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTACTGATGCTGAGCGTGAAATCTGTC8950.14490477570521673No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8880.1437714422639469No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8800.14247620404535277No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8780.14215239449070424No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8620.139561918053516No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8460.13697144161632777No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8360.13535239384308512No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8350.13519048906576087No Hit
TTATAATACTCCTCCGGCTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT8120.1314666791873028No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8070.13065715530068145No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7950.12871429797279027No Hit
GTTTATTACTGTCAGCAGTATTATAATACTCCTCCGGCTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA7900.12790477408616896No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7900.12790477408616896No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7760.12563810720362925No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7620.12337144032108956No Hit
GCTTAGGAGGCAGTCCAACTTTCTGCTGGTACCAAGCCAAGTAGTTCTTATTATTGGAGGTGTATAAAAGACT7510.12159048777052264No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7480.12110477343854983No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC7280.11786667789206455No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7230.11705715400544324No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6850.11090477246712119No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA6760.1094476294712028No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6760.1094476294712028No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACGCTCAG6740.10912381991655427No Hit
ATTCAGTGCCAGCGGGTCTGGGACAGATTTCACGCTCAGCATCAGTAGCC6590.1066952482566903No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGCC6520.10556191481542046No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6360.10297143837823222No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6250.10119048582766532No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT6220.10070477149569253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)