FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666023
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAAA22260.33422269200913485No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22040.330919502779934No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20630.3097490627200562No Hit
ATATATTACTGTGCGAGGCGGGCCACAATACAAAGTCATTTTTTCGGTAT19000.2852754334309776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15680.23542730506303836No Hit
GACTTACAGTCCCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15500.23272469569369228No Hit
GTCTACAACCTGAAGATTTTGGATCTTACTTCTGTCAACAGACTTACAGTCCCCCGTGGACGTTCGGCCAGGGGA15500.23272469569369228No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15330.2301722312893098No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14780.22191425821630784No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13780.20689976171994062No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13200.1981913537520476No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12410.18632990151991746No Hit
CTGTAAGTCTGTTGACAGAAGTAAGATCCAAAATCTTCAGGTTGTAGACTGGTGATGGTGAGAGTGAATTCTGTC12110.1818255525710073No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATCAGCACATATTTAAATTGGTATGTGCAGAAA12070.18122497271115262No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11920.17897279823669754No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11920.17897279823669754No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT11780.1768707687272061No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11520.17296699963815063No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11270.16921337551405882No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11120.16696120103960374No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11060.1660603312498217No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11050.16591018628485804No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10830.16260699705565723No Hit
GAATTCACTCTCACCATCACCAGTCTACAACCTGAAGATTTTGGATCTTACTTCTGTCAACAGACTTACAGTCCC10660.1600545326512748No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10600.15915366286149277No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9780.14684177573447163No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9620.14443945629505286No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9570.1436887314702345No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9540.14323829657534348No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9270.13918438252132434No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCGAACGTC9270.13918438252132434No Hit
GATCTATGCTGCATCCAGTTTGCAGACTGGGGTCCCATCAAGTTTCAGTGGCAGTGGATCTGGGACAGAATTCA9170.13768293287168762No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9170.13768293287168762No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9030.1355809033621962No Hit
GTGCAGAAACCAGGGAAAGCCCCTAAATTCCTGATCTATGCTGCATCCAG9020.13543075839723254No Hit
GGTTAAGCCTTCGGAGACCCTGTCCCTCACCTGCGGTGTCAATGGTGGGT9010.13528061343226885No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8910.13377916378263213No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8840.13272814902788643No Hit
CACCATCACCAGTCTACAACCTGAAGATTTTGGATCTTACTTCTGTCAACAGACTTACAGTCCCCCGTGGACGTT8750.13137684434321337No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCGAACGTCCACGGGGGA8750.13137684434321337No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT8590.12897452490379463No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC8540.12822380007897627No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8500.12762322021912156No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8230.12356930616510241No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8080.12131713169064731No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7990.11996582700597427No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7980.11981568204101059No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7750.11636234784684614No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA7670.11516118812713676No Hit
GTTGTAGACTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAAACTTGATGGGACCCCAGT7600.11411017337239104No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7550.11335944854757268No Hit
CTTCTACTGAGACGGTGACTCGACTCCTGAGGGACGGGTTGTAGTTGGAG7550.11335944854757268No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7500.11260872372275432No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7490.11245857875779065No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAAACTTGATGGGACCCCA7410.11125741903808127No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7400.11110727407311759No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7360.11050669421326291No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC7280.10930553449355353No Hit
CAGTAATATATAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAAAACTGGTTCTTGGACGCTTCTA7150.10735364994902578No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7090.10645278015924374No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7050.10585220029938906No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6930.10405046071982499No Hit
CAGTAGAAGCGTCCAAGAACCAGTTTTCCCTGAGGCTGAGCTCTGTGACCGCCGCGGACACGGCTATATATTA6930.10405046071982499No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6910.10375017078989765No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG6820.10239886610522458No Hit
ATGCTGCATCCAGTTTGCAGACTGGGGTCCCATCAAGTTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCA6760.10149799631544257No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6750.10134785135047887No Hit
TATTTAAATTGGTATGTGCAGAAACCAGGGAAAGCCCCTAAATTCCTGATCTATGCTGCATCCAGTTTGCAGA6730.10104756142055155No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG6720.10089741645558786No Hit
CAGTTACACCAGGGGGGCGCAGGACTGGTTAAGCCTTCGGAGACCCTGTC6700.10059712652566054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)