FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666023
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24040.3609484957726685No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22660.34022849060768173No Hit
GTAATATATAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAAA21020.31560471635363946No Hit
ATATATTACTGTGCGAGGCGGGCCACAATACAAAGTCATTTTTTCGGTAT18490.2776180402178303No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17260.2591502095272986No Hit
GACTTACAGTCCCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15320.23002208632434615No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14810.22236469311119883No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14460.21710961933747033No Hit
GTCTACAACCTGAAGATTTTGGATCTTACTTCTGTCAACAGACTTACAGTCCCCCGTGGACGTTCGGCCAGGGGA13890.208551356334541No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13860.20810092143964998No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12890.19353685983817376No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12170.18272642236078934No Hit
CTGTAAGTCTGTTGACAGAAGTAAGATCCAAAATCTTCAGGTTGTAGACTGGTGATGGTGAGAGTGAATTCTGTC11990.18002381299144324No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11940.17927308816662488No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATCAGCACATATTTAAATTGGTATGTGCAGAAA11680.1753693190775694No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11240.1687629406191678No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT10560.1585530830016381No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10520.15795250314178338No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10300.1546493139125826No Hit
GAATTCACTCTCACCATCACCAGTCTACAACCTGAAGATTTTGGATCTTACTTCTGTCAACAGACTTACAGTCCC10140.15224699447316384No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9930.1490939502089267No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9890.14849337034907203No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9720.14594090594468961No Hit
CACCATCACCAGTCTACAACCTGAAGATTTTGGATCTTACTTCTGTCAACAGACTTACAGTCCCCCGTGGACGTT9540.14323829657534348No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9480.14233742678556147No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9440.14173684692570676No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9410.14128641203081577No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9220.13843365769650598No Hit
GGTTAAGCCTTCGGAGACCCTGTCCCTCACCTGCGGTGTCAATGGTGGGT8970.13468003357241415No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8940.13422959867752315No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8850.13287829399285012No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8830.13257800406292275No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCGAACGTC8750.13137684434321337No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8710.1307762644833587No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8530.12807365511401256No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8490.12747307525415788No Hit
GTGCAGAAACCAGGGAAAGCCCCTAAATTCCTGATCTATGCTGCATCCAG8480.1273229302891942No Hit
GATCTATGCTGCATCCAGTTTGCAGACTGGGGTCCCATCAAGTTTCAGTGGCAGTGGATCTGGGACAGAATTCA8380.1258214806395575No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCGAACGTCCACGGGGGA8300.12462032091984812No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT7990.11996582700597427No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7900.11861452232130121No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC7900.11861452232130121No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7850.11786379749648285No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7700.11561162302202778No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7700.11561162302202778No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7660.11501104316217307No Hit
GTTGTAGACTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAAACTTGATGGGACCCCAGT7530.11305915861764533No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA7510.11275886868771798No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7360.11050669421326291No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC7320.1099061143534082No Hit
CTTCTACTGAGACGGTGACTCGACTCCTGAGGGACGGGTTGTAGTTGGAG7170.10765393987895314No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7160.10750379491398945No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7100.10660292512420742No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7060.10600234526435273No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAAACTTGATGGGACCCCA6990.10495133050960703No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6980.10480118554464335No Hit
TATTTAAATTGGTATGTGCAGAAACCAGGGAAAGCCCCTAAATTCCTGATCTATGCTGCATCCAGTTTGCAGA6950.10435075064975233No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6870.10314959093004296No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6840.10269915603515194No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6830.10254901107018828No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6810.10224872114026093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)