FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025129
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGG39480.38512226266157723No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG36020.3513704128943772No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27300.2663079475851332No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT26960.2629912918276627No Hit
GTAATACACAGCCGTGTCCTCGGCTCCCAGACTGTTCATTTGCAAATACA26780.2612354152501783No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAG24340.2374335327553898No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC23880.2329462926129297No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21580.21051009190062908No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21530.2100223484068834No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC21120.20602285175816895No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG20330.19831650455698746No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC19580.1910003521508025No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC19370.1889518294770707No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG18070.1762704986396834No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16880.16466220348853655No Hit
GACCTAGGACGGTGACCTCGGTCCCAGCTCCGAAGACATAACCATTCAGG16840.16427200869354003No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16730.16319897300729957No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16630.16222348601980824No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16500.16095535293606952No Hit
GTGTATTACTGTACAAGACGCTCCCACCTAGGGGGGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC16240.15841908676859207No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT16070.1567607588898568No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15640.15256616484364405No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15270.14895686298992614No Hit
ACCTAGGACGGTGACCTCGGTCCCAGCTCCGAAGACATAACCATTCAGGC14950.1458353046299539No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAGCTCCGAAGACATAACCATTCAGGCTGTCATCCCATG14900.14534756113620823No Hit
CTCCTGTGCAGCCTCTGGGTTCACCTTCAGTAACTACTGGATGCACTGGGTCCGCCTAGTTCCAGGGAAGGGG14570.14212845407748684No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14550.14193335667998858No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT14540.14183580798123943No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14140.1379338600312741No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTC14010.13666572694753537No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13960.1361779834537897No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13710.1337392659850614No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC13450.13120299981758393No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13140.1281789901563608No Hit
GCCCTGGGATTCCCAGCTGTTTCTGCTTGCTGATCAGGACTGCACACAGA12740.12427704220639547No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12720.12408194480889721No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12430.12125303254517236No Hit
GCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAGCTGGGACCGAGGT12420.12115548384642325No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12340.12037509425643017No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC12120.11822902288394924No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC12030.11735108459520703No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAGCTGGGAC12000.11705843849895965No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT11830.11540011062022439No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT11770.11481481842772957No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG11680.1139368801389874No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11550.11266874705524867No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11400.11120551657401165No Hit
CCCCTGAGGGCCGCTGATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA11390.11110796787526253No Hit
GTCTTGTACAGTAATACACAGCCGTGTCCTCGGCTCCCAGACTGTTCATT11230.10954718869527641No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG11050.10779131211779201No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11040.10769376341904287No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC10900.10632808163655501No Hit
GTCTGGGAGCCGAGGACACGGCTGTGTATTACTGTACAAGACGCTCCCAC10880.10613298423905675No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC10840.10574278944406022No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC10830.10564524074531108No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACCCCC10830.10564524074531108No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10770.10505994855281628No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT10660.10398691286657581No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10540.10281632848158623No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC10490.10232858498784056No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10460.10203593889159315No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT10420.10164574409659663No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10330.10076780580785442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT750.054.7345121
AACTGTA1156.850314E-944.96568370
GGTATCA7100.044.3272441
CGTATGC1800.038.4492641
TCGTATG1850.037.369540
ATTACAC952.419256E-1035.9814453
ATCTCGT1750.035.42510637
GTATCAA17000.035.4164471
TGCCGTC2000.034.72263745
ATGCCGT2000.034.69513344
TCTCGTA1850.033.5597138
CGAGACG2050.033.3519123
ACGAGAC2100.032.5562322
TATACAC2150.031.7975543
GTATGCC2300.031.61399542
ACGGGTC652.472762E-531.5529616
ACCGCTC450.00416066231.33341456
ACAGGTA553.4225726E-431.0991541
TTATACA2200.031.07642
AGCGTCA553.4382756E-431.0748833
Produced by FastQC (version 0.11.5)