FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800088
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGG40850.510568837427883No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32250.40308066112727603No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29810.3725840157582666No Hit
CAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA22200.27746947835738067No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21520.268970413254542No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19240.24047354790972994No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18990.2373488916219216No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17500.2187259401465839No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17180.21472638009818917No Hit
GTCTCCAGGCATCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGGGTGTTAGCAG16640.20797712251652317No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16280.20347761746207915No Hit
GTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTT15890.19860315365309816No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15030.18785433602303747No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15020.18772934977152514No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15020.18772934977152514No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14120.17648058713541512No Hit
ACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACAGACTTCACTCTCA14070.17585565587785343No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13460.16823149453560107No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12610.1576076631570527No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12300.15373308936017038No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12060.15073341932387438No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAATGTA11880.14848366679665237No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11570.14460909299977004No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAAGTG11460.14323424423313436No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11130.13910969793322733No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11010.13760986291507935No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCC10850.135610082890882No Hit
CTCCTGCTGGGCCAGTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACC10760.134485206627271No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10750.13436022037575868No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10660.13323534411214766No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10550.131860495345512No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10310.12886082530921597No Hit
GTCTGGCACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT10130.12661107278199396No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10090.12611112777594463No Hit
CTCCAGGCATCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC10000.12498625151233364No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9870.1233614302426733No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT9830.12286148523662398No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9750.1218615952245253No Hit
CTTCCATACTGCTGACAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTG9710.12136165021847597No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9690.1211116777154513No Hit
ATGTATCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGCTAAGGAGTTGCTGCTAACAC9610.12011178770335264No Hit
GGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGGCCAGG9600.11998680145184029No Hit
GAGTGAAGTCTGTGCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA9460.11823699393066762No Hit
GTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGGC9210.11511233764285929No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC8870.11086280509143993No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8850.11061283258841528No Hit
GTATTACTGTGTGAGACGTGGCAGCAACTTTTTTCATTGGGGGCCCTACTACTTTGACTCGTGGGGCCAGGGAAC8850.11061283258841528No Hit
ATGTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGAC8840.11048784633690295No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8830.1103628600853906No Hit
GTGCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT8510.10636330003699593No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC8500.1062383137854836No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8300.10373858875523694No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8210.10261371249162593No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTT8140.10173880873103958No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8110.10136384997650259No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8070.10086390497045325No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8030.10036395996440392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG150.002473772368.1511317
ATTACGT200.007602024451.3250632
AATTACG200.007615236651.3025231
TATCTCG1850.044.5514136
GGTATCA8550.043.8700681
CGTATGC1950.042.56701741
TCGTATG1950.042.51307740
ATCTCGT2000.041.27504737
CGGACTC2250.041.00857528
GACGGAC2100.040.64489426
ATGCCGT2200.039.45460544
GAATATA350.001427277238.970291
AGACGGA2200.038.7925425
ACGAGAC2200.038.7537222
CGAGACG2250.037.89726323
GCCGTCT2300.037.84281546
TCTCCGA2300.037.0432911
TACGTAT700.001905687736.7391570
TATACAC2350.036.2483333
ATGGCAA2900.035.47228270
Produced by FastQC (version 0.11.5)