FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800088
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGG36680.45844957054723984No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31140.38920718720940695No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28210.3525862155162932No Hit
CAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA21420.2677205507394187No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20930.2615962244153143No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19960.24947255801861795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19730.24659787423383428No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17880.22347541770405258No Hit
GTCTCCAGGCATCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGGGTGTTAGCAG17170.21460139384667687No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16900.21122676505584384No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15870.1983531811500735No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15670.1958534561198268No Hit
GTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTT15630.1953535111137775No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14000.1749807521172671No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13460.16823149453560107No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13450.16810650828408874No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13330.16660667326594075No Hit
ACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACAGACTTCACTCTCA13230.1653568107508174No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12720.1589825119236884No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAATGTA12320.15398306186319505No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12200.15248322684504706No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12170.15210826809051006No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12150.15185829558748537No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12030.15035846056933738No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10940.13673495915449302No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10590.13236044035156133No Hit
GGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGGCCAGG10540.13173550909399964No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAAGTG10520.131485536590975No Hit
GTCTGGCACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT10280.12848586655467897No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCC10240.12798592154862964No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10230.1278609352971173No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10160.12698603153653099No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10140.12673605903350632No Hit
ATGTATCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGCTAAGGAGTTGCTGCTAACAC9930.12411134775174731No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9910.12386137524872264No Hit
CTCCTGCTGGGCCAGTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACC9750.1218615952245253No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9650.12061173270940197No Hit
CTCCAGGCATCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC9590.11986181520032796No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9490.11861195268520462No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9320.11648718640949494No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC9270.11586225515193328No Hit
GAGTGAAGTCTGTGCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA9240.11548729639739629No Hit
GTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGGC9080.11348751637319895No Hit
CTTCCATACTGCTGACAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTG9030.11286258511563729No Hit
ATGTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGAC8920.11148773634900161No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8800.10998790133085361No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA8670.10836308006119327No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8380.1047384787673356No Hit
GTATTACTGTGTGAGACGTGGCAGCAACTTTTTTCATTGGGGGCCCTACTACTTTGACTCGTGGGGCCAGGGAAC8320.10398856125826159No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8100.10123886372499025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA552.6077032E-854.6879369
TCGATTA200.00758198151.35933733
TATTCGT306.7663606E-445.3531236
GGTATCA7700.044.2138141
TACGTAT650.00116580340.6031170
GTATCAA21250.039.091251
GTATAAT601.4698606E-534.0446361
TATCAAC24700.033.739422
ATCAACG25600.032.54923
TCAACGC26150.031.734554
AACGCAG26250.031.6176076
CAACGCA26300.031.5574995
CTCGCTA1101.207809E-930.97873923
CCGAAAC500.004525771430.79816268
ACGCAGA27400.030.2905927
ACACCGG450.00495334830.2354186
CGCAGAG28100.029.5360228
AGAGTAC28550.028.95133811
GGTACGT954.4799417E-628.36672868
GCAGAGT29350.028.1622099
Produced by FastQC (version 0.11.5)