FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116048
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40330.3613643857611859No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39480.3537482258827577No Hit
GTCTGGGACAGACTTCACTTTCACCATCAGCAGCCTGGAGCCTGAAGACTTTGCAGTTTATTATTGTCAGCAGCG34530.30939529482602895No Hit
GTAGTAGACGGCCGTGTCCGCGGCAGTCACAGAGGTCAAGTTCAGGGAGA30300.2714936991957335No Hit
GTCCCAGACCCAGCGCCACTGAACCTGGCTGGGATGTCAGTGGCCCTTTT29920.26808882772067155No Hit
CTCCCAGGAGAGTGTCACAGAACAGGACAGCAAGGACAGCACCTACAGCC27280.24443393115708284No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA27160.24335870858601064No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT26960.24156667096755696No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25360.22723037001992746No Hit
GTCCTGTTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22040.19748254555359626No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21930.19649692486344672No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21150.18950797815147735No Hit
GAGTATTAGCAAGTACTTAGTCTGGTACCAACAGAAACCTGGCCAGCCTCCCAGACTCCTCCTGTATGATGCCTC20930.1875367367711783No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20490.1835942540105802No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAAGTACTTAGTCTGGTACCAACAGAAACCTGGCCAGCCTCCCAGACT20290.18180221639212651No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGTTCT20210.18108540134474505No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18450.16531547030235258No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18410.16495706277866184No Hit
GTGCAGCCACAGTTCGGTTGATCTCCACCTTGGTCCCAGGGCCGAAAGTT18070.16191059882729059No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18020.16146258942267716No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAAGTACTTAGTCTGGTA17890.16029776497068227No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17210.15420483706793975No Hit
GGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACT16980.152143993806718No Hit
CTCCCAGACTCCTCCTGTATGATGCCTCCAAAAGGGCCACTGACATCCCA16380.14676788095135693No Hit
GTATTAGCAAGTACTTAGTCTGGTACCAACAGAAACCTGGCCAGCCTCCC16320.14623026966582084No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16030.14363181511906298No Hit
GTCTCTGGTGACTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGGTCCG15870.14219818502430004No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15870.14219818502430004No Hit
GAACAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15870.14219818502430004No Hit
GTCTACTACTGTGCGAGAGACCGCGTCGGGCCCCACCCGGGTCTCTATTA15080.13511963643140795No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14800.1326107837655728No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATCTCCACCTTGGTCC14740.1320731724800367No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14720.1318939687181913No Hit
GTCTTCAGGCTCCAGGCTGCTGATGGTGAAAGTGAAGTCTGTCCCAGACC14660.13135635743265522No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14440.12938511605235617No Hit
GTATGATGCCTCCAAAAGGGCCACTGACATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTTCACTTT14160.12687626338652103No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGTTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14100.1263386521009849No Hit
GTTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14080.12615944833913953No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14000.12544263329175806No Hit
CTGCTACTCTGGCTCCCAGATATTACCGGAGAAATTGTTTTGACACAGTC13220.11845368657978869No Hit
ATACAGGAGGAGTCTGGGAGGCTGGCCAGGTTTCTGTTGGTACCAGACTAAGTACTTGCTAATACTCTGACTGGC13040.11684085272318037No Hit
GCCCTGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTG12940.11594483391395352No Hit
CCTCCTGTATGATGCCTCCAAAAGGGCCACTGACATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTT12930.11585523203303084No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12630.1131671756053503No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12430.11137513798689662No Hit
GTCTGGTACCAACAGAAACCTGGCCAGCCTCCCAGACTCCTCCTGTATGATGCCTCCAAAAGGGCCACTGACATC12350.11065832293951515No Hit
GTCTGGGAGGCTGGCCAGGTTTCTGTTGGTACCAGACTAAGTACTTGCTA12230.10958310036844293No Hit
GTTCAGTGGCGCTGGGTCTGGGACAGACTTCACTTTCACCATCAGCAGCC11940.1069846458216851No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11810.1058198213696902No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11780.10555101572692215No Hit
GTCCTTGCTGTCCTGTTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11540.10340057058477771No Hit
CCACTGAACCTGGCTGGGATGTCAGTGGCCCTTTTGGAGGCATCATACAG11340.10160853296632402No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11340.10160853296632402No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11280.10107092168078792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)