FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116048
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40490.36279801585594884No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35050.31405459263400853No Hit
GTCTGGGACAGACTTCACTTTCACCATCAGCAGCCTGGAGCCTGAAGACTTTGCAGTTTATTATTGTCAGCAGCG33130.2968510314968532No Hit
GTCCCAGACCCAGCGCCACTGAACCTGGCTGGGATGTCAGTGGCCCTTTT27070.2425522916577065No Hit
CTCCCAGGAGAGTGTCACAGAACAGGACAGCAAGGACAGCACCTACAGCC27050.2423730878958611No Hit
GTAGTAGACGGCCGTGTCCGCGGCAGTCACAGAGGTCAAGTTCAGGGAGA26300.23565294682665977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26010.23305449227990194No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT25140.22525912863962838No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA24790.22212306280733446No Hit
GTCCTGTTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23010.2061739280030966No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22390.2006186113858902No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAAGTACTTAGTCTGGTACCAACAGAAACCTGGCCAGCCTCCCAGACT21320.19103121012716298No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21310.1909416082462403No Hit
GAGTATTAGCAAGTACTTAGTCTGGTACCAACAGAAACCTGGCCAGCCTCCCAGACTCCTCCTGTATGATGCCTC20300.18189181827304918No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19760.17705331670322424No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAAGTACTTAGTCTGGTA19300.17293163018078075No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGTTCT17550.157251301019311No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17420.1560864765673161No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17230.1543840408297851No Hit
GTGCAGCCACAGTTCGGTTGATCTCCACCTTGGTCCCAGGGCCGAAAGTT16990.15223359568764067No Hit
GAACAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16990.15223359568764067No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16600.14873912233165598No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16540.14820151104611987No Hit
GGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACT16400.1469470847132023No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16190.14506544521382594No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16100.14425902828552176No Hit
GTATTAGCAAGTACTTAGTCTGGTACCAACAGAAACCTGGCCAGCCTCCC15540.13924132295385144No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15270.13682207216893896No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14990.13431321950310382No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14900.13350680257479963No Hit
GTCTCTGGTGACTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGGTCCG14700.131714764956346No Hit
GTCTACTACTGTGCGAGAGACCGCGTCGGGCCCCACCCGGGTCTCTATTA14650.13126675555173256No Hit
GTTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14480.1297435235760469No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGTTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14370.1287579028858974No Hit
CTCCCAGACTCCTCCTGTATGATGCCTCCAAAAGGGCCACTGACATCCCA14000.12544263329175806No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATCTCCACCTTGGTCC13980.12526342952991268No Hit
GTCTTCAGGCTCCAGGCTGCTGATGGTGAAAGTGAAGTCTGTCCCAGACC13560.12150015053115995No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13210.11836408469886599No Hit
GCCCTGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTG13020.11666164896133499No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12890.1154968245093401No Hit
GTATGATGCCTCCAAAAGGGCCACTGACATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTTCACTTT12760.1143320000573452No Hit
CTGCTACTCTGGCTCCCAGATATTACCGGAGAAATTGTTTTGACACAGTC12710.11388399065273179No Hit
CCTCCTGTATGATGCCTCCAAAAGGGCCACTGACATCCCAGCCAGGTTCAGTGGCGCTGGGTCTGGGACAGACTT12450.111554341748742No Hit
ATACAGGAGGAGTCTGGGAGGCTGGCCAGGTTTCTGTTGGTACCAGACTAAGTACTTGCTAATACTCTGACTGGC12440.1114647398678193No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12400.11110633234412857No Hit
GTCTGGGAGGCTGGCCAGGTTTCTGTTGGTACCAGACTAAGTACTTGCTA12390.11101673046320587No Hit
GTCTGGTACCAACAGAAACCTGGCCAGCCTCCCAGACTCCTCCTGTATGATGCCTCCAAAAGGGCCACTGACATC12060.10805986839275732No Hit
GTCCTTGCTGTCCTGTTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11940.1069846458216851No Hit
GTTCAGTGGCGCTGGGTCTGGGACAGACTTCACTTTCACCATCAGCAGCC11620.10411738563215918No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11270.10098131979986524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGT150.002400362268.6726436
GTGGTAT2950.044.9887471
GGTATCA10800.042.5374371
TACCGAT7000.037.7390570
GTATCAA27250.037.5890461
TGGTATC3550.034.4891472
TATCAAC30700.033.013092
ATCAACG31850.031.7086283
TCAACGC32750.030.8372464
CAACGCA32850.030.7488825
AACGCAG33000.030.6118586
TATAGTG450.004957538630.2312245
CGCAGAG34750.029.2660148
ACGCAGA34600.029.1962817
AGAGTAC34950.029.0012211
GCAGAGT36650.027.7488129
TACACCG756.7937115E-527.2081015
AACTTAT659.1119227E-426.30663131
GTCTTAC659.3787647E-426.1768861
CCGTTAA1058.6567525E-725.92874516
Produced by FastQC (version 0.11.5)