FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139022
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT61570.5405514555469517No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35830.3145681119416482No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32860.28849311075642087No Hit
GATCTATGGTGCGTCCAGTTTGCAGAGCGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA31710.27839672982611396No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25570.22449083511995377No Hit
CCATAGATCAGTACCTCAGGGGCTTTCCCTGGTTTGTGTTGATACCAATT25110.22045228274783102No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25110.22045228274783102No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGA23680.2078976525475364No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22740.19964495856972034No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAACACCTATTTAAATTGGTATCAACACAAA20570.18059352672731518No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20230.17760850975661577No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20210.1774329205230452No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19490.17111170811450527No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18900.1659318257241739No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18730.16443931723882418No Hit
GTCTCCATCCTCCCTGACTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAACAC18220.15996179178277503No Hit
ATTTACTACTGTCAACAGATTTACACTTCTTTGTTCACTTTTGGCCAGGGGACCAAGCTGGAAATCAGACGAA18010.15811810483028424No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17940.15750354251278728No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17880.15697677481207561No Hit
GTCTCTGGTGGCTCCATCAGCGGTTACTCCTGGACCTGGATCCGGCAGCC17390.15267483858959705No Hit
CCTGAGGATTGTGCAATTTACTACTGTCAACAGATTTACACTTCTTTGTT17380.15258704397281175No Hit
ATCCTCAGGTTGCAGACTGCCGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGAC17340.15223586550567064No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16430.14424655537821043No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16220.14240286842571961No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16020.14064697609001406No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCA15360.13485253138218578No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15320.13450135291504467No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15000.1316919251779158No Hit
GTAGTAAATTGCACAATCCTCAGGTTGCAGACTGCCGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA14740.1294092651414986No Hit
GTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG14650.1286191135904311No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14620.12835572974007525No Hit
GTACTGATCTATGGTGCGTCCAGTTTGCAGAGCGGGGTCCCATCAAGGTT14580.12800455127293414No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCC14430.126687632021155No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14070.12352702581688502No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13870.12177113348117946No Hit
GTCTATTACTGTGCGAGACGGACACATATGGGCTTTAATTACTTCTTTGA13790.12106877654689725No Hit
GATTTACACTTCTTTGTTCACTTTTGGCCAGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCAT13680.1201030357622592No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13160.11553771568942478No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13020.1143085910544309No Hit
GTGTAAATCTGTTGACAGTAGTAAATTGCACAATCCTCAGGTTGCAGACTGCCGATGGTGAGAGTGAAATCTGTC12950.11369402873693396No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12860.11290387718586646No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12830.11264049333551064No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12460.10939209251445538No Hit
GATTTCACTCTCACCATCGGCAGTCTGCAACCTGAGGATTGTGCAATTTA12210.10719722709482346No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC12160.10675825401089706No Hit
GGCCAAAAGTGAACAAAGAAGTGTAAATCTGTTGACAGTAGTAAATTGCA12140.10658266477732652No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC11950.10491456705840625No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11870.10421221012412403No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11650.10228072855484793No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11580.10166616623735099No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11570.10157837162056572No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11420.10026145236878656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC11850.043.061493
CTTATAC12000.042.8198931
TTATACA11950.042.701142
CGTATGC12150.042.68997241
GTATGCC12550.041.69914242
TCGTATG12200.041.5726140
ATGCCGT12700.041.17069644
CCGTCTT12750.041.06339647
AAAGGGG12200.040.86106568
ATCTCGT11100.040.7285737
CTTGAAA13450.040.6208857
TACACAT12700.040.184715
TCTCCGA12600.039.9724511
ACGAGAC12650.039.82319622
CACGAGA12650.039.55076221
CGTCTTC13300.039.4759348
ATCTCCG12800.039.3478810
AATCTCG11550.039.0542936
ATACACA13100.038.9525644
GGCAATC11700.038.9047933
Produced by FastQC (version 0.11.5)