FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708999
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22290.3143869032255335No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21140.29816685213942473No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGCATTAGTTGGGATAGTGGT18260.257546202462909No Hit
GTATTATTGTGCAAAAGATAGGGGTGTATTACCGAAGTGGGGACTTGACTACTGGGGCCAGGGAGCCCTGGTCAC18010.25402010440071143No Hit
GAGTTACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCAT15940.2248240124457157No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT14630.20634725859980055No Hit
CAATAATACAGGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATA14040.19802566717301434No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13700.1932301738084257No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13270.1871652851414459No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12720.1794078694046113No Hit
GTCTGAGACCTGAGGACACGGCCCTGTATTATTGTGCAAAAGATAGGGGT12670.1787026497921718No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT12240.172637761125192No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11940.16840644345055494No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA11840.16699600422567593No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11800.16643182853572433No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11780.1661497406907485No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGTACATTAGCAG11710.16516243323333318No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11700.16502138931084528No Hit
GTACATTAGCAGCTATTTAAATTGGTATCAGCAGAATTCAGGGAAAGTCCCTATCCTCCTGATCTATGGTGCATC11140.1571229296515228No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11040.15571249042664376No Hit
ACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGA10930.15416100727927684No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10900.15373787551181312No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10550.14880133822473657No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10550.14880133822473657No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCAAAATCATCTACGGTGAAC10480.14781403076732125No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC10370.14626254761995433No Hit
GTCCCTATCCTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT10340.14583941585249063No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10300.145275240162539No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10180.1435827130926842No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10060.14189018602282938No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9950.14033870287546243No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC9930.14005661503048664No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9820.13850513188311972No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGTACATTAGCAG9580.13512007774341006No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT9560.13483798989843426No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9200.1297604086888698No Hit
CTCCTGTGCAGCCTCTGGGTTCACCGTAGATGATTTTGCCATGCACTGGGTCCGGCAAGGTCCAGGGAAGGGC9140.1289141451539424No Hit
TCCATAGACTATGCGGACTCTGTGAAGGGCCGCTTCAGCATCTCCAGAGACAACGCCAAGAACTCCCTGTATCTG9070.12792683769652707No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8980.12665744239413596No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCATCCAGATGACCCAGTC8940.12609326670418433No Hit
AGTCAGTACATTAGCAGCTATTTAAATTGGTATCAGCAGAATTCAGGGAA8760.12355447609940212No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8760.12355447609940212No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8660.1221440368745231No Hit
GGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC8560.12073359764964407No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8470.11946420234725295No Hit
ATAATACAGGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATACA8420.11875898273481345No Hit
GTCTATGGAACCACTATCCCAACTAATGCCTGAGACCCACTCCAGGCCCTTCCCTGGACCTTGCCGGACCCAGTG8290.11692541174247073No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8280.11678436781998282No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8180.1153739285951038No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8060.11368140152524897No Hit
CCATAGATCAGGAGGATAGGGACTTTCCCTGAATTCTGCTGATACCAATT8040.11339931368027317No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7920.11170678661041834No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7900.11142469876544255No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCAT7870.11100156699797885No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7850.11071947915300305No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7770.10959112777309982No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7720.10888590816066032No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7700.1086038203156845No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAA7680.1083217324707087No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTG7680.1083217324707087No Hit
GTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGT7670.1081806885482208No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7630.1076165128582692No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7500.10578294186592647No Hit
GGCCTGGAGTGGGTCTCAGGCATTAGTTGGGATAGTGGTTCCATAGACTA7490.10564189794343858No Hit
GTGCCAGATGTGTCATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA7450.10507772225348695No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGCTCCCTGG7400.10437250264104744No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGGTA7360.10380832695109585No Hit
GCCCTGTATTATTGTGCAAAAGATAGGGGTGTATTACCGAAGTGGGGACT7340.10352623910612005No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7330.10338519518363212No Hit
ATACAGGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATACAGGG7300.10296206341616843No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGGTACTGTAACTCTGTTGACAG7200.10155162419128942No Hit
GGCTTGGTACAGCCCGGCAGTTCCCTGAGACTCTCCTGTGCAGCCTCTGGGTTCACCGTAGATGATTTTGCCATG7140.100705360656362No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7130.1005643167338741No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7130.1005643167338741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGACG200.006974304652.45530748
AATGTCG200.007482643251.53030836
ACGCGTA1700.048.03273827
CGTATGC1850.046.7709641
GACGCGT1750.046.6241126
ATGCCGT1800.046.3306444
CTTATAC1950.045.274621
TCGTATG1850.044.82275440
TAGTAAT2000.044.43436432
ATCTCGT1550.044.3746737
CGTAGTA2000.044.3241730
CGCGTAG1850.044.17567428
GCCGTCT2000.043.57026346
AATCTCG1650.041.64064836
ACGAGAC2050.041.4038722
GTATGCC2050.040.56923342
GCGTAGT2100.040.55541629
TATACAC2450.040.1729353
CACGAGA2150.039.4781121
TCTCCGA2150.039.46140311
Produced by FastQC (version 0.11.5)