FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708999
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27570.3888580942991457No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22880.3227084946523197No Hit
GTATTATTGTGCAAAAGATAGGGGTGTATTACCGAAGTGGGGACTTGACTACTGGGGCCAGGGAGCCCTGGTCAC18340.2586745538428122No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGCATTAGTTGGGATAGTGGT17650.24894252319114696No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17270.24358285413660666No Hit
GAGTTACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCAT14960.2110017080419013No Hit
CAATAATACAGGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATA13050.18406231884671206No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12840.18110039647446613No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12440.17545863957495003No Hit
GTCTGAGACCTGAGGACACGGCCCTGTATTATTGTGCAAAAGATAGGGGT12440.17545863957495003No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT12380.17461237604002264No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11650.1643161696984058No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA11500.16220051086108725No Hit
GTACATTAGCAGCTATTTAAATTGGTATCAGCAGAATTCAGGGAAAGTCCCTATCCTCCTGATCTATGGTGCATC11330.1598027641787929No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11280.15909754456635342No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGTACATTAGCAG10970.15472518296922844No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10610.14964760175966396No Hit
ACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGA10560.14894238214722447No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10090.14231331779029308No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10040.14160809817785358No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCAAAATCATCTACGGTGAAC9990.14090287856541406No Hit
GTCCCTATCCTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT9890.139492439340535No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9780.13794095619316812No Hit
CTCCTGTGCAGCCTCTGGGTTCACCGTAGATGATTTTGCCATGCACTGGGTCCGGCAAGGTCCAGGGAAGGGC9700.1368126048132649No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9650.13610738520082538No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGTACATTAGCAG9630.13582529735584958No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9570.13497903382092216No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9460.13342755067355527No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9370.13215815537116413No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC9300.1311708479137488No Hit
ATAATACAGGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATACA9220.1300424965338456No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9210.1299014526113577No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9200.1297604086888698No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9200.1297604086888698No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9030.12736266200657548No Hit
AGTCAGTACATTAGCAGCTATTTAAATTGGTATCAGCAGAATTCAGGGAA8910.12567013493672063No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT8810.12425969571184163No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC8740.12327238825442632No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8470.11946420234725295No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCATCCAGATGACCCAGTC8430.11890002665730134No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8290.11692541174247073No Hit
TCCATAGACTATGCGGACTCTGTGAAGGGCCGCTTCAGCATCTCCAGAGACAACGCCAAGAACTCCCTGTATCTG8190.1155149725175917No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8140.1148097529051522No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8090.11410453329271268No Hit
GCCCTGTATTATTGTGCAAAAGATAGGGGTGTATTACCGAAGTGGGGACT7990.11269409406783365No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7950.11212991837788205No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7900.11142469876544255No Hit
CCATAGATCAGGAGGATAGGGACTTTCCCTGAATTCTGCTGATACCAATT7870.11100156699797885No Hit
GGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC7840.11057843523051512No Hit
GTCTATGGAACCACTATCCCAACTAATGCCTGAGACCCACTCCAGGCCCTTCCCTGGACCTTGCCGGACCCAGTG7800.11001425954056353No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7800.11001425954056353No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTG7720.10888590816066032No Hit
ATACAGGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATACAGGG7680.1083217324707087No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCAT7670.1081806885482208No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7640.1077575567807571No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGCTCCCTGG7610.10733442501329338No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7440.10493667833099905No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7410.10451354656353536No Hit
GTAGTAAGTTGCAGAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA7410.10451354656353536No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAA7400.10437250264104744No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7130.1005643167338741No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGGTA7090.10000014104392249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)