FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904728
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33680.37226658177927513No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32500.3592239877620677No Hit
GAGTTACATTATGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT26070.28815290341406474No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20530.22691902980785383No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTAAAATCTGTC20020.22128197646143372No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18790.20768673015536163No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18620.20580771237322157No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18440.20381816413330855No Hit
ACATTATGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGA18000.19895482399129905No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17430.19265458789824125No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA17360.19188087469383064No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16970.18757018684068583No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTTA16800.18569116905854577No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16790.18558063860077284No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16790.18558063860077284No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16030.1771803238100291No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14830.16391666887727582No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14790.16347454704618405No Hit
GCCTTGTACTACTGTGTAAAAGGGGATGGCAACAGTTGGTTCGTGAAAAC14650.16192712063736284No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTATCAC14500.16026916377076866No Hit
GTAGTACAAGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGCAGAAACA14310.15816908507308272No Hit
GCATAATGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTAAAAT14290.15794802415753684No Hit
GATTTTACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGTTACATTATG13660.15098460531784139No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13350.14755816112688014No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13260.14656338700692362No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTATCACCTACTTAAATTGGTATCAGCAGAAA12970.14335800373150825No Hit
GGCCAAAAGTGTACGGCATAATGTAACTCTGTTGACAGTAGTAAGTTGCA12900.14258429052709765No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12580.13904731587836344No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12540.13860519404727167No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT12310.13606299351849396No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCGTGATGACCCAGTC12170.13451556710967275No Hit
GAGTAAAATCTGTCCCAGATCCACTGCCACTGAACCTGGATGGGACCCCA12060.13329973207417037No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12050.1331892016163974No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12000.1326365493275327No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11970.13230495795421388No Hit
GTCTGAGAGTTGAGGACACGGCCTTGTACTACTGTGTAAAAGGGGATGGC11960.13219442749644092No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11920.13175230566534915No Hit
GTACAAGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGCAGAAACAGGG11830.13075753154539266No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11730.1296522269676632No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11670.12898904422102556No Hit
CAGTAGTACAAGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGCAGAAA11660.1288785137632526No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT11640.12865745284770672No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11600.12821533101661495No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11540.12755214826997727No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11510.12722055689665843No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11450.1265573741500208No Hit
ACTTACTACTGTCAACAGAGTTACATTATGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA11380.12578366094561017No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11330.12523100865674547No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11220.12401517362124308No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10980.12136244263469241No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC10980.12136244263469241No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTAAAATCTGTCCCAGATCCACTGCCA10790.11926236393700648No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10770.1190413030214606No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10760.11893077256368764No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10450.11550432837272638No Hit
GTACTACTGTGTAAAAGGGGATGGCAACAGTTGGTTCGTGAAAACGACTT10350.11439902379499695No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10170.11240947555508395No Hit
GTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTTACT9980.11030939685739802No Hit
CTCCTGTGCAGCCTCTGGATTCACATTTGATGATTATGCCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGC9950.10997780548407919No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9920.10964621411076035No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG9900.10942515319521448No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9850.10887250090634976No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9760.10787772678639326No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT9740.10765666587084738No Hit
GCACTGAACACAGAGGACTCACCATGGACTTGGGACTGAGGTGGATTTTC9710.10732507449752854No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9580.10588817854648026No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCGTGATGACCCAGTCTCCATCCTCC9380.10367756939102139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)