FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904728
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32730.3617661882908454No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26890.2972164009514462No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26140.2889266166184754No Hit
GAGTTACATTATGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT25790.2850580505964223No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20990.23200343086540925No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTAAAATCTGTC19280.21310272258623586No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19000.21000786976859342No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17980.1987337630757532No Hit
ACATTATGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGA17760.19630209300474838No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17150.1895597350805988No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTTA17130.1893386741650529No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA16880.1865754127207293No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16440.1817120725787198No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15380.16999584405478774No Hit
GATTTTACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGTTACATTATG14190.15684271957980742No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTATCAC13910.15374786676216498No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13890.1535268058466191No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13720.15164778806447904No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13580.15010036165565782No Hit
GCCTTGTACTACTGTGTAAAAGGGGATGGCAACAGTTGGTTCGTGAAAAC13510.14932664845124724No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13470.14888452662015544No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13370.14777922204242602No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13300.1470055088380154No Hit
GTAGTACAAGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGCAGAAACA13210.14601073471805892No Hit
GGCCAAAAGTGTACGGCATAATGTAACTCTGTTGACAGTAGTAAGTTGCA13160.14545808242919417No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13010.14380012556260002No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12920.14280535144264353No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12850.14203163823823292No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTATCACCTACTTAAATTGGTATCAGCAGAAA12690.14026315091386582No Hit
GCATAATGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTAAAAT12480.13794201130063402No Hit
GAGTAAAATCTGTCCCAGATCCACTGCCACTGAACCTGGATGGGACCCCA12330.13628405443403985No Hit
GTCTGAGAGTTGAGGACACGGCCTTGTACTACTGTGTAAAAGGGGATGGC12240.13528928031408335No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCGTGATGACCCAGTC12030.13296814070085153No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11760.12998381834098202No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11710.12943116605211732No Hit
ACTTACTACTGTCAACAGAGTTACATTATGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA11640.12865745284770672No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11620.1284363919321608No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11600.12821533101661495No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11410.126115252318929No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11400.12600472186115605No Hit
GTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTTACT11170.12346252133237837No Hit
GTACAAGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGCAGAAACAGGG11120.12290986904351363No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11020.12180456446578419No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10840.1198150162258712No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10800.11937289439477942No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10650.11771493752818527No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG10560.11672016340822876No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10470.11572538928827228No Hit
CTCCTGTGCAGCCTCTGGATTCACATTTGATGATTATGCCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGC10460.11561485883049934No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTAAAATCTGTCCCAGATCCACTGCCA10380.11473061516831579No Hit
CAGTAGTACAAGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGCAGAAA10250.1132937192172675No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT10180.1125200060128569No Hit
GCACTGAACACAGAGGACTCACCATGGACTTGGGACTGAGGTGGATTTTC9990.11041992731517097No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9930.10975674456853331No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9870.10909356182189564No Hit
GTACTACTGTGTAAAAGGGGATGGCAACAGTTGGTTCGTGAAAACGACTT9720.10743560495530147No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC9290.1026827952710649No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9200.1016880211511084No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9100.10058271657337897No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9090.10047218611560602No Hit
TTGTACTACTGTGTAAAAGGGGATGGCAACAGTTGGTTCGTGAAAACGACTTTTGACTACTGGGGCCAGGGAACC9070.10025112520006013No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9070.10025112520006013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)