FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960200
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43590.45396792334930225No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38850.40460320766506974No Hit
GAGTTACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT33250.3462820245782129No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28420.29598000416579884No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26740.2784836492397417No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26140.2722349510518642No Hit
GTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTTCGGCCAAGGGG24760.2578629452197459No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24060.2505727973338888No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23920.24911476775671734No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23840.24828160799833368No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23700.2468235784211623No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21710.2260987294313685No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTC21020.2189127265153093No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGTAACTATTTAAATTGGTATCAGCAGAAA20990.2186002916059154No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20470.21318475317642158No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20260.21099770881066443No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19550.20360341595500936No Hit
GATCTATTCTGCTTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCA19090.19881274734430326No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18790.19568839825036452No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18500.19266819412622369No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18170.18923141012289107No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTTGGCCGAACGTC18140.18891897521349718No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17930.18673193084774006No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGTAA17320.18037908769006455No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16650.17340137471360134No Hit
ACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGA16600.17288064986461155No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15520.16163299312643198No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15500.16142470318683608No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14810.15423870027077694No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14770.15382212039158508No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14660.15267652572380755No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCC14490.15090606123724223No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT13940.1451780878983545No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13930.14507394292855655No Hit
CACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTT13700.1426786086232035No Hit
ACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAA13620.14184544886481984No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13580.141428868985628No Hit
GTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTA13570.14132472401583004No Hit
GAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT13560.14122057904603208No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13350.13903353468027493No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13340.13892938971047697No Hit
GATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATC13210.13757550510310354No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13140.1368464903145178No Hit
GTATTATAGTGGGAGCAAAATGTACAATCCGTCCCTCGAGAGCCGAGTCA12970.1350760258279525No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTG12640.13163924182461986No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA12100.1260154134555301No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12020.12518225369714645No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11870.12362007915017705No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11740.12226619454280359No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGA11620.12101645490522808No Hit
GTTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTA11350.11820454072068319No Hit
GGATAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAAT11220.11685065611330972No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11180.11643407623411789No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11150.11612164132472401No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11150.11612164132472401No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10930.11383045198916893No Hit
GTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA10920.11372630701937098No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10910.11362216204957301No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTGATGGGACCCCA10910.11362216204957301No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10320.10747760883149343No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10280.1070610289523016No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10270.10695688398250365No Hit
GTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCT10230.1065403041033118No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10130.10549885440533222No Hit
CCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAG10110.10529056446573631No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10070.10487398458654447No Hit
GTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCACT10070.10487398458654447No Hit
GCCCCTAACCTCCTGATCTATTCTGCTTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTAGTGGAT10030.10445740470735264No Hit
GTCTCTGGTGGTTCCATCAGCAATAAAAATTGGTGGAGTTGGGTCCGCCA9930.10341595500937305No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9930.10341595500937305No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9900.10310352009997917No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9840.10247865028119142No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9790.10195792543220161No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9610.10008331597583836No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTACTA9610.10008331597583836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2000.047.6556851
CGTATGC2150.046.60117741
TCGTATG2150.046.53474440
CACGAGA2200.044.87295521
GGCAATC2000.044.45318633
CGAGACA2400.043.9772123
ATGCCGT2400.041.9420544
GTATGCC2450.040.97290842
TACACAT2700.040.3266335
AATCTCG2250.039.6363336
GTATCAA26250.038.90261
TTATACA2650.038.5175362
TATACAC2650.038.5175363
CGAACTA801.8262654E-938.3907529
ACGAGAC2600.037.97337722
ATCTCCG2600.037.9516310
CCGTCTT2600.037.51823847
ATCTCGT2400.037.20398337
GCAATCT2400.037.07731234
AGGCAAT2700.036.70657332
Produced by FastQC (version 0.11.5)