FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960200
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41740.4347011039366798No Hit
GAGTTACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32790.3414913559675068No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30560.318267027702562No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28010.2917100604040825No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27750.28900229118933557No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26250.27338054571964177No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25130.2617163091022704No Hit
GTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTTCGGCCAAGGGG24170.25171839200166635No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23230.2419287648406582No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22300.23224328264944802No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21930.22838991876692355No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20720.21578837742137055No Hit
GATCTATTCTGCTTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCA20470.21318475317642158No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTC19360.20162466152884814No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGTAACTATTTAAATTGGTATCAGCAGAAA19010.19797958758591958No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18390.19152259945844616No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18190.18943970006248698No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18130.18881483024369922No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18120.18871068527390128No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17720.18454488648198292No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTTGGCCGAACGTC17560.1828785669652156No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17450.18173297229743804No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGTAA17230.17944178296188293No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17230.17944178296188293No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16650.17340137471360134No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16420.17100604040824827No Hit
ACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGA16170.16840241616329932No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15720.16371589252239116No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14690.15298896063320142No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14410.15007290147885857No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14400.1499687565090606No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14290.14882316184128308No Hit
GTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTA14100.14684440741512186No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14010.14590710268694024No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13730.14299104353259737No Hit
CACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTT13710.14278275359300147No Hit
GATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATC13500.14059570922724432No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCC12830.13361799625078108No Hit
ACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAA12790.13320141637158925No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12710.1323682566132056No Hit
GAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT12360.12872318267027702No Hit
GTATTATAGTGGGAGCAAAATGTACAATCCGTCCCTCGAGAGCCGAGTCA12280.12789002291189336No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12100.1260154134555301No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA12060.12559883357633825No Hit
GTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCACT12000.12497396375755052No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTG11990.12486981878775255No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11900.12393251405957093No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGA11760.1224744844823995No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11470.1194542803582587No Hit
GTTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTA11470.1194542803582587No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11230.11695480108310768No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11060.11518433659654238No Hit
GTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA11000.11455946677775464No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10910.11362216204957301No Hit
GGATAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAAT10810.11258071235159342No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10690.1113309727140179No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC10620.1106019579254322No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10520.10956050822745261No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10390.10820662362007916No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTGATGGGACCCCA10220.10643615913351387No Hit
GTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCT10140.10560299937513018No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10040.10456154967715059No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9970.10383253488856488No Hit
AGTCAGACCATTAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATT9760.10164549052280776No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9730.10133305561341388No Hit
CCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAG9710.10112476567381795No Hit
CAATAATACACGGCCGTGTCCGCGGCGGCCACAGACCTCAACTCCAGGAACAACTGATTCTTGGACTTGTCTA9670.10070818579462613No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9640.10039575088523224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)