FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180908
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGTCTCAGGGAGA40950.3467670639880499No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38500.32602031656996144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37250.315435241356651No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32900.27859917961433067No Hit
GTCTCTGGTGGCTCCATGACCGATTACTCCTGGACCTGGATCCGGCAGCC29930.2534490409075051No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27020.22880698581091838No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26460.2240648721153553No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24540.2078061965877105No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAACAGCTATTTAAATTGGTATCAGCACAAA23770.20128579025631127No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23120.1957815511453898No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22980.19459602272149906No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGTTCCCAGATGGGTC22650.1918015628651851No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21980.1861279625508507No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21930.1857045595423183No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21770.18434966991501456No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21430.1814705294569941No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21330.18062372343992927No Hit
GTCTCCATCCTCCCTGACTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAACAG21040.17816798599044126No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21030.17808330538873476No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20820.17630501275289862No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20710.1753735261341273No Hit
GATCTATGGTGCGTCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA19960.16902248100614103No Hit
GTCTATTACTGTGCGAGACGGACACATATAGGCTTTAATTACTTCTTTGA19930.16876843920102158No Hit
CATATAGGCTTTAATTACTTCTTTGACTCCTGGGGCCAGGGAGCCCTGGT19760.16732886897201138No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19080.1615705880559705No Hit
CCATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTGTGCTGATACCAATT18990.16080846264061216No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT18360.1554735847331037No Hit
GTGTACATCTGTTGACAGTAGTAAATTGCAAAATCCTCAGGTTGCAGACTGCCGATGGTGAGAGTGAAATCTGTC18140.15361061149556104No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18080.15310252788532214No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17640.14937658141023685No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17460.14785233057952016No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17390.14725956636757478No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17270.14624339914709697No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16860.14277149447713117No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCA16780.14209404966347927No Hit
CACCTGCACTGTCTCTGGTGGCTCCATGACCGATTACTCCTGGACCTGGATCCGGCAGCCCCCCGGGAAGGGA16750.14184000785835985No Hit
GATGTACACTTCTTTGTTCACTTTTGGCCAGGGGACCAAGCTGGAAATCAAACGAACTGTGGCTGCACCAT16720.1415859660532404No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16630.14082384063788203No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG16330.13828342258668752No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16190.13709789416279675No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15760.13345662828941798No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15620.1322710998655272No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15550.1316783356535818No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAGCTTGGTCC15430.13066216843310402No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15320.1297306818143327No Hit
GATTTCACTCTCACCATCGGCAGTCTGCAACCTGAGGATTTTGCAATTTA15200.12871451459385488No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15000.12702090255972523No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14740.12481920691535665No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14660.1241417621017048No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14250.12066985743173897No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14230.120500496228326No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14120.11956900960955467No Hit
GGACTGGAGTGGATTGGCTTTATCCTTTACACTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGACTCAG14110.1194843290078482No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13770.11660518854982775No Hit
CAGTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGTCTCAGGGA13640.11550434072764347No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13580.11499625711740458No Hit
GTCTGCAACCTGAGGATTTTGCAATTTACTACTGTCAACAGATGTACACTTCTTTGTTCACTTTTGGCCAGGGGA13480.11414945110033975No Hit
GCCTATATGTGTCCGTCTCGCACAGTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGTCTCAGGGAGAA13430.11372604809180731No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13410.11355668688839435No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13150.11135499124402579No Hit
GGCCAAAAGTGAACAAAGAAGTGTACATCTGTTGACAGTAGTAAATTGCA13090.11084690763378689No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13060.11059286582866742No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13000.11008478221842853No Hit
CCTTTACACTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGACTCAGCTTATCGGTGGACACGTCCAAGAA12960.10974605981160261No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12910.10932265680307018No Hit
GGTTTGTGCTGATACCAATTTAAATAGCTGTTAATGGTCTGACTTGCCCGGCAAGTGATGGTGACT12890.10915329559965721No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12880.10906861499795073No Hit
CTCCTGGACCTGGATCCGGCAGCCCCCCGGGAAGGGACTGGAGTGGATTGGCTTTATCCTTTACACTGGGAGCAC12860.10889925379453777No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12810.10847585078600534No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12750.10796776717576645No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12700.10754436416723402No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGTTCC12520.10602011333651733No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12490.10576607153139789No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12470.1055967103279849No Hit
GTGTAAAGGATAAAGCCAATCCACTCCAGTCCCTTCCCGGGGGGCTGCCG12420.10517330731945249No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12300.10415714009897468No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA12170.1030562922767904No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12160.10297161167508392No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12150.10288693107337744No Hit
GCACAAACCAGGGAAAGCCCCTGAACTCCTGATCTATGGTGCGTCCAGTTTGCAGAGTGGGGTCCCATCAAGGTT12080.10229416686143204No Hit
GGATAAAGCCAATCCACTCCAGTCCCTTCCCGGGGGGCTGCCGGATCCAGGTCCAGGAGTAATCGGTCATGGAGC11990.1015320414460737No Hit
CCTTGGGGCTGGTCGGGGATGCTGCGGAGACGGTGACCAGGGCTCCCTGG11960.10127799964095424No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11880.10060055482730239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT453.0529918E-853.61200339
CGTATGC3150.050.48748841
TCGTATG3150.050.41168240
GTATGCC3650.044.57797242
ATGCCGT3550.043.98360444
TATACAC3750.043.6106953
CTTATAC3700.043.3085371
GTATCAA25700.043.114921
TTATACA3750.042.7021372
ACGAGAC3850.042.4833522
CCGTCTT3800.042.1630547
ATCTCGT3300.041.66672537
ATCTCCG3850.041.59475310
TAAGACA3900.041.23083531
CCCTAAG4000.040.97176728
CACCGAT12200.040.5651270
CACGAGA4050.040.38540321
CCCACGA4050.040.38198519
ACACATC4150.038.5879026
TACACAT4150.038.5879025
Produced by FastQC (version 0.11.5)