FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949654
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30550.32169611247886076No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30050.31643103698820835No Hit
GTTGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAAGATTGGCAAATATTTAAATTGGTATCAGCAGAAA20900.2200801555092697No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20130.21197193925366503No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19940.20997121056721713No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19810.20860229093964752No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18750.19744033089946444No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17450.18375113462376824No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16690.17574821987797662No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16540.1741686972307809No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16200.1705884458971373No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15960.16806120966162413No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15860.16700819456349367No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15140.15942648585695424No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14060.14805392279714508No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13710.1443683699536884No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13570.14289414881630572No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13130.13826088238453163No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCGAGGTGCCAGATGTGACGT12890.13573364614901848No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT12580.132469299344814No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACATC12480.13141628424668353No Hit
GTAATATATGGCCGTGTCCGCGGCCGTCACAGAAGTCAGCTTAAGGGAGAACTGATTCTGGGACGTGTCGATTGA12400.13057387216817914No Hit
CCCCTAAACTCCTGATCTCTTATGCAACCGATTTGCAAGGTGGGGTCCCA12290.12941555556023562No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12130.12773073140322685No Hit
ACTTACTACTGTCAACACTATTTCAGGGCCCTCGGGATGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11840.12467698761864847No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11520.12130733930463096No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACATCCCGAGGGCC11500.12109673628500485No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11330.11930661061818305No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11320.11920130910837001No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGAAGAGCGTTGATGGCGAGAGTGA11300.1189907060887439No Hit
GTCTGCATCTGTTGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA11120.11709527891210904No Hit
ATTTCAGGGCCCTCGGGATGTTCGGCCAAGGGACCAAGGTGGAAATCAAA11030.11614756532379161No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10960.11541045475510028No Hit
GATTTCACTCTCGCCATCAACGCTCTTCAACCTGAAGATTTTGCAACTTACTACTGTCAACACTATTTCAGGGCC10880.1145680426765959No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10870.11446274116678286No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10840.11414683663734371No Hit
GTTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAATTTAAATATTTGCCA10750.11319912304902627No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10270.10814465057799999No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTTGGGGACAGAGTCACCATCACTT9920.1044590977345433No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCGAGGTGCCAGATGTGACGTCCAGATCCAGCTGACCCAGTCTCCGTC9510.10014173583220837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA352.481001E-548.55969210
TATCTCG2450.043.39617536
ATGCCGT2650.041.92302344
TCGTATG2650.041.6564440
CGAGACG2600.040.5457523
CGTATGC2800.039.48814841
ACGAGAC2800.038.8620822
ATCTCGT2700.038.1482937
CCGTCTT2950.037.855147
GTATCAA31700.037.5402451
TCCTATC2800.036.62611833
GACGGAC2900.036.37243326
ACTCCTA3100.036.32826631
GTATGCC3050.036.32529442
CTTATAC3150.035.619821
CACGAGA3050.034.55812521
CTCCTAT3250.033.6210832
CGGACTC3150.033.51399628
CGTCTTC3400.032.89821648
GTGGTAT3750.032.6407051
Produced by FastQC (version 0.11.5)