FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229252
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCGCGGCAGTCAGAGAGTTCAGCTTCAGGGAGA51760.42106907289961704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49240.40056880118966653No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46790.38063798147165917No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37260.30311116028283863No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30990.25210453186165244No Hit
CCTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCA30500.24811836791805097No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30000.24405085368988622No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28740.233800717834911No Hit
GTAGTACCCTGGGGTACACCTTTGGCCAGGGGACCAAGCTAGAGATCAAA25670.20882618047397933No Hit
GGGTACTACGGTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT25530.20768727649009316No Hit
CCGTAGTACCCTGGGGTACACCTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCAT25390.20654837250620703No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT25120.20435191482299805No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24580.19995899945658008No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG24330.19792524234249773No Hit
GTACTACGGTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC24060.19572878465928872No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23670.19255612356132024No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT23290.189464812747915No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23190.18865130990228204No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23050.1875124059183959No Hit
GGCCAAAGGTGTACCCCAGGGTACTACGGTGCTGACAGTAATAAACTGCA22440.18255003856003488No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21550.1753098632339016No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21540.1752285129493383No Hit
GTATATTACTGTGCGAGGACATATAATTATGATACTAGTGGGTTTTCACCACACGGGTACTGGGGCCAGGGAACC21470.17465906095739522No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTA21440.17441501010370533No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20450.1663613319319391No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19480.15847035432929943No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT18610.15139287957229275No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18600.15131152928772945No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18320.14903372131995718No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18190.14797616762063434No Hit
GTCTCTGGCGGCTCCATCAGCAGTGGTGGGCACTTCTGGAACTGGATCCG18060.1469186139213115No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17680.14382730310790626No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17580.14301380026227334No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT17460.14203759684751377No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17370.1413054442864441No Hit
GTTTATTACTGTCAGCACCGTAGTACCCTGGGGTACACCTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAA17020.13845818432672877No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA16920.13764468148109582No Hit
GTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG16610.13512282265963366No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15960.12983505416301946No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15720.12788264733350035No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCACCGTAGTACCCTGGGGTACACCTTTGGCCAGGGGA15510.12617429135767116No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA15140.12316433082882924No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15070.12259487883688616No Hit
GTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC14860.12088652286105697No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14480.11779521204765174No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14370.1169003589174555No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13980.11372769781948697No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCCCCTGGCCAAAGGTG13950.11348364696579709No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13910.1131582458275439No Hit
CAGTAATATACGGCCGTGTCCGCGGCAGTCAGAGAGTTCAGCTTCAGGGAGAACTGGTTCTTAGACGTGTCTA13790.11218204241278437No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13680.1112871892825881No Hit
ACCCTGGGGTACACCTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT13630.11088043785977163No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13530.1100669350141387No Hit
GGGGTACACCTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGG13430.10925343216850573No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13390.10892803103025255No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13390.10892803103025255No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13350.10860262989199937No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCC13300.1081958784691829No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13190.10730102533898662No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13120.10673157334704357No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13120.10673157334704357No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13060.10624347163966379No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13040.10608077107053722No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12980.10559266936315742No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12960.10542996879403083No Hit
CACCTGCACTGTCTCTGGCGGCTCCATCAGCAGTGGTGGGCACTTCTGGA12640.10282675968800539No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC12550.10209460712693574No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12490.10160650541955596No Hit
GAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC12350.10046760143566982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG250.001203932381.711670
GGTATCA10250.046.3321531
GTATCAA28600.041.3940281
TACTGAT16200.039.5107570
ATCAACG33450.034.9595223
TATCAAC33550.034.9591942
AACGCAG34250.034.045376
TCAACGC34350.033.9448744
CAACGCA34550.033.7497525
TCTACTG11450.032.86557468
GTCTACT11150.032.5663267
ACGCAGA36150.032.162227
CGCAGAG36650.031.908428
TGTCTAC11250.031.7462866
AGAGTAC37050.031.4724411
GCAGAGT37450.031.0457749
AATGGTA3950.031.0297270
CGGTCTA450.00456104130.75106842
GACAGGT2650.030.721591
GACGTGT11800.030.60890862
Produced by FastQC (version 0.11.5)