FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494469
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48710.3259351649314907No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43440.2906718038313274No Hit
GTCTCTGGTGGCTCCATCAATTCTTACTACTGGACCTGGATCCGGCAGCC36000.24088823521933206No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA35990.24082132182066004No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31420.21024189862753928No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29030.1942495963449225No Hit
GTTTTACTGTCAGCAATATGGTAGCTCGCCTTGGACGTTCGGCCAAGGGA28720.19217528098608938No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28690.19197454079007326No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28660.19177380059405716No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT27410.18340962576005257No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26330.1761829787034726No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25330.16949163883626894No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24770.1657444885106349No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24700.16527609471993063No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG24270.16239881857703303No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC23300.1559082189058455No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23170.155038344723109No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT22630.15142502119481904No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22100.1478786110652011No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22090.14781169766652905No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21770.14567046890902388No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21530.144064547340895No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21400.1431946731581585No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20830.13938060943385241No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20780.13904604244049226No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT20560.13757394766970743No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20540.13744012087236335No Hit
CAGTAAAACATTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20450.13683790028431503No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20430.13670407348697095No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20110.13456284472946578No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAATTCTTACTACTGGACCTGGATCCGGCAGCCCCCAGGGAAGGGG19980.1336929705467293No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCA19960.1335591437493852No Hit
ATAATAGACGGCCGTGTCTGCGGCGGTCACAGAACTCAACTTCAGGGAGA19790.13242161597196062No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19730.1320201355799284No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19510.1305480408091436No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19330.12934359963304692No Hit
CAATAATAGACGGCCGTGTCTGCGGCGGTCACAGAACTCAACTTCAGGGAGAACTGATTCTTGGACGTGTCCA19100.12780459146359008No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA19090.12773767806491804No Hit
CTCCCAGACTCCTCATCTACGGTGCATCCAACAGGGCCACTGGCATCCCA19080.12767076466624602No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18800.125797189503429No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18740.12539570911139675No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18580.12432509473264416No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17920.11990881042028975No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAATGTTTTACTGTCAGCAATATGGTAGCTCG17720.118570542446849No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17420.11656314048668791No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17360.11616166009465569No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17130.11462265192519885No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17110.11448882512785478No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17090.1143549983305107No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17080.11428808493183866No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16760.11214685617433348No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC16510.11047402120753257No Hit
GTGTTAGCAGCACCTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGACTCCTCATCTACGGTGCAT16270.1088680996394037No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16210.10846661924737147No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15790.10565625650314593No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACC15650.10471946892173742No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15630.10458564212439336No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15510.1037826813403289No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15470.10351502774564075No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15470.10351502774564075No Hit
CTATTATTGTGCGAGACATTATCAGCAGTGGCTGGCACTAGGATACTTTGCCTACTGGGGCCAGGGAATCCTGGT15400.1030466339549365No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15370.10284589375892039No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA15320.10251132676556021No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15230.10190910617751188No Hit
GTCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACCAGCAGAGACCTGGC15160.10144071238680763No Hit
GCAGAGACCTGGCCAGGCTCCCAGACTCCTCATCTACGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTT15130.10123997219079152No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15060.10077157840008727No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15050.10070466500141523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTA252.752047E-454.429424
CGTATGC4200.046.93892341
TCGTATG4150.045.77534540
GACTAGG4250.044.89783526
GAGACTA4350.043.82897624
AGACTAG4400.043.34398325
ACTAGGC4450.042.12835727
ATGCCGT4700.042.1115944
ATCTCGT4300.041.58373337
GGTATCA14100.041.2742961
GTATGCC5000.040.8580242
ACGAGAC4700.040.5556122
CGAGCCC4800.040.39683515
CTTATAC4750.040.123341
CACGAGA4850.039.99776521
TTACGGA554.9663904E-639.88394566
TATACAC4950.039.8599243
CGAGACT4850.039.30789623
CCCACGA5050.038.4072719
TACACAT5250.038.2314645
Produced by FastQC (version 0.11.5)