FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053542
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT78840.7483327669898305No Hit
CTATAATATTGGTGACAATAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC60310.5724498880917894No Hit
CTCTTATACACCTCCGACAACAGGAACTACTTGGCTTGGTTGCAGCAGAA51510.488922131248683No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGCAACCAAGCCAAGTAGTTCCTG48240.4578839761490287No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43300.41099453083028487No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43160.4096656801532355No Hit
CTCCTAAGGTGCTCATTTATTGGGCATCCACCCGGGAATCCGGGGTCCCT39270.3727426149123623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30870.29301157428939706No Hit
GTATAAGAGACTCCGGCTGGACTTGCAGTTGATGGTGGCCCGCTCGCCCAGAGACACAGCCAGAGAGTCTGGAGA30360.2881707611087171No Hit
CTTATACACCTCCGACAACAGGAACTACTTGGCTTGGTTGCAGCAGAAAC29750.28238076887300173No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29590.28086208238494526No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26370.2502985168128086No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26200.2486849124192486No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25160.2388134502468815No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25120.23843377862486737No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24550.23302345801116614No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24510.232643786389152No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23890.22675887624793314No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23480.22286724212228845No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20960.1989479299353989No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20190.19163925121162706No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19870.1886018782355141No Hit
GTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATA19550.18556450525940113No Hit
GTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGACAACAGGAACTA18440.17502861774850934No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17440.16553682719815632No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17030.16164519307251157No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16960.16098076773398687No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16600.1575637231358598No Hit
AACCAGGACAGCCTCCTAAGGTGCTCATTTATTGGGCATCCACCCGGGAA16280.15452635015974683No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT15970.15158389508913742No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15810.1500652086010809No Hit
CTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGC15700.14902111164054208No Hit
CTCTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGA15440.14655324609745032No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15410.14626849238093972No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14870.1411429254837491No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14310.13582752277555143No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14230.1350681795315232No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTATTGTCACCAATATTATAGTGCT13720.13022736635084317No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13650.12956294101231844No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13540.1285188440517796No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13510.12823409033526903No Hit
CATTTATTGGGCATCCACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA13480.12794933661875843No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13330.1265255680362055No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13160.12491196364264548No Hit
GGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGACA13090.12424753830412077No Hit
GTCAGGGACCCCGGATTCCCGGGTGGATGCCCAATAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGCAA12970.12310852343807842No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12620.11978639674545485No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12550.11912197140693014No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12550.11912197140693014No Hit
GTGTATAAGAGACTCCGGCTGGACTTGCAGTTGATGGTGGCCCGCTCGCC12440.11807787444639133No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12320.11693885958034896No Hit
GTCCTGGTTTCTGCTGCAACCAAGCCAAGTAGTTCCTGTTGTCGGAGGTG12180.11561000890329953No Hit
ATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA12160.11542017309229247No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12150.11532525518678895No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT12120.11504050147027836No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC12110.11494558356477483No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11940.11333197917121482No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11740.11143362106114423No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11680.11086411362812303No Hit
GGATCTCTGGTGCCTACGGGGACGTCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGCGGG11670.1107691957226195No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11660.11067427781711599No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11560.10972509876208067No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11550.10963018085657715No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11460.10877591970704537No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTAAAGCCGGGGGGGTCCCTTAGACTCTCCTGTGCAGC11360.10782674065201008No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11340.10763690484100301No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11320.10744706902999597No Hit
GGCTGAGGATGTGGCAGTTTATTATTGTCACCAATATTATAGTGCTCCGT11320.10744706902999597No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAATGTCTGG11280.10706739740798184No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11220.10649788997496065No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11150.10583346463643596No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11100.10535887510891828No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10900.10346051699884769No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10890.10336559909334417No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10880.10327068118784063No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10730.10184691260528768No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10710.10165707679428063No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10700.1015621588887771No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC10660.10118248726676297No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10620.10080281564474887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAA350.00529366856.24020470
TCGTATG2350.052.79340
ACGAGAC2900.045.5840522
CGTATGC2900.044.03053741
GTGGTAT2500.043.382541
GTATGCC2950.043.35342842
CCCTAAG3100.042.7446428
CACGAGA3050.042.22485421
TCTCCGA3050.042.2048111
TAAGACA3150.042.17456431
ATCTCCG3100.041.52409410
ACCCTAA3200.041.39113627
CCGTCTT3150.040.93786247
ATGCCGT3150.040.74685744
GACCCTA3250.040.7407826
CGTCTTC3100.040.57691248
ATCTCGT2800.040.4305937
CCCACGA3150.039.8009219
GTATCAA23100.039.0278551
GGTATCA8650.038.790391
Produced by FastQC (version 0.11.5)