FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352050
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47220.34924743907399874No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45890.33941052475869976No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32960.24377796679116898No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32780.24244665507932397No Hit
CTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAGGGTGGAGGTCAAACGAACTGTGG30010.22195924706926518No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29780.2202581265485744No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26160.19348396878813653No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26010.19237454236159907No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25400.18786287489367998No Hit
GTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAGGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTT24610.1820198957139159No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24160.17869161643430345No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23620.17469768129876853No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23300.17233090492215525No Hit
GTCTCCAGGCATCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCTGGGCCAGTCAGGGTGTTAGCAG23250.17196109611330943No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT21240.15709478199770718No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21150.1564291261417847No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20340.15043822343848232No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAATCTA20330.15036426167671313No Hit
CAGTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTGCCAGACCCACTGCCA20190.14932879701194482No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20010.14799748530009985No Hit
ACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACGGACTTCACTCTCA19810.14651825006471655No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19510.1442993972116416No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19060.14097111793202913No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18270.1351281387522651No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18150.1342405976110351No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17430.12891535076365518No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17240.12751007729004105No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17170.1269923449576569No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16760.12395991272512112No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16380.12114936577789283No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCC16300.12055767168373951No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15960.11804297178358789No Hit
CTTCCATACTGCTGACAGTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTG15610.1154543101216671No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15390.11382715136274546No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15370.11367922783920713No Hit
CTCCTGCTGGGCCAGTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACC15360.11360526607743797No Hit
GGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14870.10998113975074886No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14800.1094634074183647No Hit
ATGTATCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGGAGTTGCTGCTAACAC14720.10887171332421139No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14320.10591324285344476No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14180.10487777818867647No Hit
GTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAGCAGAAGCCTGGC14040.10384231352390813No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13940.1031026959062165No Hit
GAGTGAAGTCCGTGCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA13880.1026589253356015No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTCTGACGCAGTC13850.102437040050294No Hit
GTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTGCCAGACCCACTGCCA13750.10169742243260234No Hit
ATCTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGAC13720.10147553714729485No Hit
CTCCAGGCATCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGC13650.1009578048149107No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCCCTCCGCCGAAAGTG13610.10066195776783403No Hit
GGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAGCAGAAGCCTGGCCAGG13600.10058799600606487No Hit
GACCAGGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13530.10007026367368071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3750.045.9780740
CGTATGC3950.043.72263741
GGCGAAT4500.041.59113732
ACGAGAC4750.040.58563622
GGTATCA17600.040.1643561
GCGAATC4200.039.74946633
ATCTCGT3850.039.1718637
CGAATCT4100.039.09485234
GACTAAG4850.039.08473226
GCCGTCT4400.038.8565546
GAGACTA4900.038.66300224
CTTATAC4900.038.654421
TATACAC4900.038.6344033
TAAGGCG4950.038.373329
ATCTCCG4850.038.337110
TCTCCGA4900.037.94590811
CACGAGA5000.037.87572521
ACTAAGG5100.037.8535727
AGACTAA5050.037.52153825
GTATGCC4750.037.15339342
Produced by FastQC (version 0.11.5)