FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352050
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46310.3425169187530047No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46040.34051995118523726No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42030.31086128471580193No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31370.2320180466698717No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30450.22521356458710845No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27740.20516992714766463No Hit
CTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAGGGTGGAGGTCAAACGAACTGTGG27550.2037646536740505No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25100.18564402204060498No Hit
GTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAGGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTT24100.17824784586368847No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23770.17580710772530603No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22930.16959431973669611No Hit
GTCTCCAGGCATCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCTGGGCCAGTCAGGGTGTTAGCAG21940.16227210532154876No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20590.15228726748271143No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20440.15117784105617396No Hit
ACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACGGACTTCACTCTCA20370.15066010872378982No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20160.14910691172663734No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAATCTA19900.14718390592063904No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19840.14674013535002403No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19630.14518693835287158No Hit
CAGTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTGCCAGACCCACTGCCA19260.14245035316741245No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19080.14111904145556747No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19040.1408231944084908No Hit
GGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG18880.13963980622018415No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17660.130616471284346No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17340.12824969490773272No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17160.12691838319588775No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16740.12381198920158278No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16620.12292444806035278No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCC16290.12048370992197034No Hit
GGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAGCAGAAGCCTGGCCAGG15920.11774712473651122No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15280.11301357198328463No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15200.11242187788913131No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15180.112273954365593No Hit
CTCCTGCTGGGCCAGTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACC14470.10702266927998226No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14420.10665286047113642No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14260.10546947228282978No Hit
ATGTATCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGGAGTTGCTGCTAACAC14130.10450796937983063No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14070.10406419880921564No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14020.10369439000036981No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14020.10369439000036981No Hit
CTTCCATACTGCTGACAGTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTG13960.10325061942975482No Hit
ATCTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGAC13860.10251100181206316No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13690.10125365186198734No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA13630.10080988129137236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)