FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840866
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTATAGTCTCCCGCGCACTTTCGGCGGAGGGAGCAAGGTGGATATCAAACGAACTGTGGCTGCACCAT31420.3736623909160318No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29760.35392083875433183No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25430.3024263081156808No Hit
CTATAAGCCTGTTGACAATAATAAGTCGCGAAATCCTCAGGCTGAAGGTTCTTGATGGTGAGAGTGAAATCTGTC24480.2911284318785633No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20990.24962360233378444No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20460.24332057664360313No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19160.22786032495070557No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18670.22203299931261344No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17270.20538349748949297No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16740.19908047179931165No Hit
GGGTATTAGGAAGTCATTAGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAGACTCCTGATCTATGCTGCATC14280.1698249185958286No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13930.16566254314004847No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12860.15293756674666356No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12690.15091584152528464No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12030.14306679066581357No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11810.14045044037932322No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT11520.13700161500167685No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11440.13605021489749852No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10810.1285579390770943No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10780.12820116403802748No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10720.12748761395989372No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10360.12320631349109132No Hit
GATCTATGCTGCATCCACTTTGCATATTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCA10190.12118458826971241No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9910.11785468790508832No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9840.1170222128139323No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT9670.1150004875925534No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9470.11262198733210761No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9430.11214628728001845No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9360.11131381218886242No Hit
CTCCTGATCTATGCTGCATCCACTTTGCATATTGGGGTCCCATCAAGATT9310.11071918712375099No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9310.11071918712375099No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9150.10881638691539437No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9150.10881638691539437No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8990.10691358670703774No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8860.10536756153774798No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8560.10179981114707932No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8530.10144303610801246No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8500.10108626106894558No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC8450.10049163600383414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1650.052.2857440
CGTATGC1900.050.9811841
CTACGCT2150.047.48521828
TACGCTG2250.045.40455629
ATCTCGT1700.044.4511637
GTATGCC2150.043.51797542
ACTACGC2350.043.4206227
ATGCCGT2350.039.92100544
CTTAACG458.137591E-539.70694758
CCGTCTT2300.039.4825347
GTATCAA25600.039.112851
ACGTTAA459.2333124E-538.86470848
AATTACG459.602742E-538.6067344
CGTTGTC2000.038.1636763
ACGCTGC2700.037.86198830
TCTCCGA2700.037.6976311
TATACAC2800.037.5633
CCCACGA2750.037.0232319
CACGAGA2800.036.37291721
ATCTCCG2800.036.3512910
Produced by FastQC (version 0.11.5)