FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840866
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTATAGTCTCCCGCGCACTTTCGGCGGAGGGAGCAAGGTGGATATCAAACGAACTGTGGCTGCACCAT28510.3390552121265457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27700.32942228607174034No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26780.3184811848736897No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24270.2886310066050952No Hit
CTATAAGCCTGTTGACAATAATAAGTCGCGAAATCCTCAGGCTGAAGGTTCTTGATGGTGAGAGTGAAATCTGTC22830.2715058047298856No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21630.2572348031672109No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19050.2265521498074604No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17510.20823769780202792No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16660.19812907169513336No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15360.1826688200022358No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15150.18017139472876773No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14240.16934921854373944No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13860.16483006804889244No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12970.15424574188990875No Hit
GGGTATTAGGAAGTCATTAGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAGACTCCTGATCTATGCTGCATC12920.1536511168247973No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11720.13938011526212263No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11650.1385476401709666No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11550.1373583900407437No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11500.13676376497563225No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10160.12082781323064554No Hit
CTCCTGATCTATGCTGCATCCACTTTGCATATTGGGGTCCCATCAAGATT9880.11749791286602147No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT9650.11476263756650881No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9630.11452478754046423No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9580.11393016247535279No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9520.11321661239721906No Hit
GATCTATGCTGCATCCACTTTGCATATTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCA9350.11119488717584015No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9140.10869746190237209No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9080.10798391182423835No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8880.10560541156379258No Hit
CCCCTAGACTCCTGATCTATGCTGCATCCACTTTGCATATTGGGGTCCCA8590.10215658618614619No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8590.10215658618614619No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8560.10179981114707932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT652.0537851E-550.1290170
CTAATGG608.357357E-443.44514570
GTAGTTT2600.040.10320770
TCGCGTC350.001335297139.50217440
AGTACGA350.001486944638.6471065
GTATCAA26100.038.2532421
GTGGTAT2950.037.859831
CAGGTAT906.3064363E-933.816222
GGGATAT800.003433131632.5838670
CCGAAAC1101.1103111E-831.90878368
ATCAACG32200.030.9788533
AACGCAG32250.030.9326656
TCAACGC32250.030.9308244
CAACGCA32550.030.6475685
TATCAAC33700.029.8024222
GACAGGT1600.029.6138421
TTAACGG2900.029.5187935
ACGCAGA33800.029.5141547
CGCAGAG33750.029.457688
CGGTACC3000.029.05622345
Produced by FastQC (version 0.11.5)