FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081034
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCGTCGCCTCTCAGAAGATTCATTTTAAGATATA46410.4293111965026077No Hit
GTTTAGGAGGTTGGCCTGGTTTCTGGAGGTACCAACCTATGTAGTTCTTA42780.39573223413879677No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37820.34985023597777687No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36270.33551211155245814No Hit
GTATATTACTGTGCAGGCTCGGGGGGAATTCCGCAGTGGTTAATCTGGGGCAAAGGGACCACAGTCACCGTCTC36030.33329201486724747No Hit
CTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT31390.29037014561984176No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAATCTTTTGTT28060.2595663041125441No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27020.24994588514329802No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24990.231167567347558No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22160.20498892726778253No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21190.1960160364983895No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20920.19351842772752753No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20700.19148333909941778No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20550.19009577867116112No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20060.1855630812721894No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19960.184638040986685No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19600.181307895958869No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19390.1793653113593097No Hit
CTACATAGGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATC19310.17862527913090614No Hit
CCTATGTAGTTCTTACTGTTGGAGCCGGAGTTGAACAAAAGATTCTGGCTGGACTTGCAATTGATGGTGGCCCT19090.1765901905027964No Hit
GTTTATTACTGTCAGCAATATTTTAGTACTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA18350.1697448923900636No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17630.16308460233443167No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17620.16299209830588124No Hit
GAACTACATAGGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGC17170.15882941702111125No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17150.15864440896401039No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17060.1578118727070564No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16990.1571643445072033No Hit
CTCCTGTGCAGCCTCTCAATTCTCCTTCAGCAACTACTGGATACACTGGGTCCGCCAAGTTCCAGGGAAGGGG16830.1556842800503962No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTTC16810.1554992719932953No Hit
CCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGAT16210.1499490302802687No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15960.1476364295665076No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15770.1458788530240492No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15750.14569384496694832No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG15710.14532382885274653No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15700.1452313248241961No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15400.1424562039676828No Hit
GTCCCTGACCGATTCAGTGGCAGCGGATCTGGGACAGATTTCACTCTCAC15350.14199368382493058No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15320.14171617173927925No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC15240.1409761395108757No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAATCTTTTGTTCAACTCCGG15120.1398660911682704No Hit
ATGTAGTTCTTACTGTTGGAGCCGGAGTTGAACAAAAGATTCTGGCTGGACTTGCAATTGATGGTGGCCCTCTCG14980.13857103476856417No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14980.13857103476856417No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14720.13616593002625266No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14590.13496337765509686No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14520.13431584945524377No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14480.13394583334104201No Hit
CCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14320.13246576888423492No Hit
GGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTC14290.1321882567985836No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14130.1307081923417765No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTAGTACT14090.1303381762275747No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13910.12867310371366675No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCA13870.12830308759946496No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13530.12515795062874988No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT13450.12441791840034633No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13300.12303035797208968No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13270.12275284588643837No Hit
ATTTTAGTACTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAA13270.12275284588643837No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13110.12127278142963127No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAGTTTAGGAGGTTGGCCTGGTTTCTGGAGGTA12960.1198852210013746No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12920.11951520488717284No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12520.11581504374515511No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12400.11470499540254978No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12370.11442748331689846No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12360.11433497928834802No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12110.11202237857458693No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12030.11128234634618338No Hit
ACATAGGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGCTCATT11990.11091233023198159No Hit
GTACTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT11960.11063481814633028No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAATCTTTTGTTCAACTCCGGCT11930.11035730606067894No Hit
GCCCTGGGATTCCCACCTGTTTCTGCTTGCTGATCAGGACTGCACACAGA11910.11017229800357804No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11890.10998728994647718No Hit
GGTTAATCTGGGGCAAAGGGACCACAGTCACCGTCTCCTCAGCATCCCCG11740.10859972951822053No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11650.10776719326126653No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11620.10748968117561519No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC11520.10656464089011077No Hit
GTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAATCTTT11490.10628712880445944No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11340.10489956837620278No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11320.1047145603191019No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC11140.10304948780519392No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC11130.10295698377664347No Hit
ATTCAGTGGCAGCGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCC10900.10082939111998328No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10880.10064438306288238No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAATTGC10830.10018186292013018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2250.055.0843440
ATCTCGT2200.054.48388337
CGTATGC2300.052.488941
GCGTATT200.00746726251.55898339
GTAAGTA200.007768863351.04519332
CGTAAGT200.007804678850.98583631
AGACGGA2450.049.73480625
GACGGAG2550.049.1459126
CGAGACG2500.048.71528623
GTGGTAT1950.048.567511
TATACAC2650.048.4751323
GTATGCC2450.047.9466442
TACACAT2750.047.9461065
ACGAGAC2550.047.75566522
CTTATAC2650.047.225681
TCTCCGA2650.047.1994711
CTACATC2550.046.76899733
ATCTCCG2750.046.71239510
CCGTCTT2550.046.52387247
ATGCCGT2600.045.36708544
Produced by FastQC (version 0.11.5)