FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081034
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCGTCGCCTCTCAGAAGATTCATTTTAAGATATA42830.396194754281549No Hit
GTTTAGGAGGTTGGCCTGGTTTCTGGAGGTACCAACCTATGTAGTTCTTA36930.3416173774367874No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36500.3376397042091183No Hit
GTATATTACTGTGCAGGCTCGGGGGGAATTCCGCAGTGGTTAATCTGGGGCAAAGGGACCACAGTCACCGTCTC35510.3284818053826244No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33150.30665085464471975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27360.253091022114013No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27260.25216598182850863No Hit
CTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT26270.24300808300201476No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23810.22025209197860568No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAATCTTTTGTT23690.21914204363600037No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21320.19721858886954527No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21110.19527600426998595No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20850.19287089952767444No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20480.18944825047130803No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCA20250.18732065781464782No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19050.17622017438859464No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18920.17501762201743887No Hit
GTTTATTACTGTCAGCAATATTTTAGTACTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA18720.17316754144642998No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18330.1695598843329627No Hit
CCTATGTAGTTCTTACTGTTGGAGCCGGAGTTGAACAAAAGATTCTGGCTGGACTTGCAATTGATGGTGGCCCT18010.16659975541934852No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17360.1605869935635697No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17350.16049448953501924No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17330.16030948147791835No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17220.1592919371638635No Hit
CTCCTGTGCAGCCTCTCAATTCTCCTTCAGCAACTACTGGATACACTGGGTCCGCCAAGTTCCAGGGAAGGGG17130.1584594009069095No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTTC16680.15429671962213953No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16620.15374169545083688No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16620.15374169545083688No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16280.1505965584801218No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC16080.14874647790911294No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15600.14430628453869165No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15520.14356625231028813No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15390.14236369993913234No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15230.14088363548232524No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15000.13875604282566506No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG14810.1369984662832066No Hit
GAGTGAAATCTGTCCCAGATCCGCTGCCACTGAATCGGTCAGGGACCCCG14780.1367209541975553No Hit
CTACATAGGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATC14750.13644344211190396No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14200.13135572054162958No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14140.13080069637032693No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13800.12765555939961185No Hit
GTACTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT13690.12663801508555697No Hit
CCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13660.12636050299990564No Hit
GTCCCTGACCGATTCAGTGGCAGCGGATCTGGGACAGATTTCACTCTCAC13590.12571297480005256No Hit
ATGTAGTTCTTACTGTTGGAGCCGGAGTTGAACAAAAGATTCTGGCTGGACTTGCAATTGATGGTGGCCCTCTCG13390.12386289422904367No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13280.12284534991498879No Hit
GAACTACATAGGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGC13270.12275284588643837No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTAGTACT13220.12229032574368613No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13010.12034774114412683No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12950.11979271697282418No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAATCTTTTGTTCAACTCCGG12500.11563003568805422No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA12460.11526001957385246No Hit
CCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGAT12410.11479749943110022No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12360.11433497928834802No Hit
ATTTTAGTACTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAA12250.11331743497429313No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12150.11239239468878869No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12100.11192987454603648No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12040.11137485037473382No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAGTTTAGGAGGTTGGCCTGGTTTCTGGAGGTA12020.11118984231763293No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11930.11035730606067894No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11820.10933976174662408No Hit
GGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTC11770.10887724160387184No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCACTGAATCGGTCAGGGACC11760.1087847375753214No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11740.10859972951822053No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11740.10859972951822053No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC11730.10850722548967008No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11610.10739717714706475No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11600.1073046731185143No Hit
GCCCTGGGATTCCCACCTGTTTCTGCTTGCTGATCAGGACTGCACACAGA11250.10406703211924881No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11200.10360451197649657No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11190.10351200794794613No Hit
GGTTAATCTGGGGCAAAGGGACCACAGTCACCGTCTCCTCAGCATCCCCG11170.10332699989084526No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCA11160.10323449586229481No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11040.10212444751968948No Hit
ATTCAGTGGCAGCGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCC11000.10175443140548772No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10920.10101439917708417No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10920.10101439917708417No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10870.10055187903433194No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10820.10008935889157973No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10820.10008935889157973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)