FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961774
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30870.32096937534181624No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30220.3142110308658791No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21990.22863999234747456No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21840.22708037439148906No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21660.22520883284430646No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20270.21075637311884082No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20190.20992457687564856No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19100.19859135306215392No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18960.1971357096365674No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17900.18611440941426988No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17180.1786282432255395No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16080.1671910448816458No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15610.16230424195289123No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15320.15928898057131924No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGTTATTTAAATTGGTATCAGCAGAAA15200.15804128620653085No Hit
GTCTCTGGTGGCTCCATCAGCGATAATAATTGGTGGACTTGGGTCCGCCA14840.15429820311216566No Hit
GAATTACTTTATCCCCCGGACGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAACTGTGGCTGCACCAT14600.15180281438258883No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTCGGTCCCTTGGCCGAACGTC14420.14993127283540625No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT14320.1488915275314159No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14120.14681203692343522No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14100.14660408786263715No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13620.14161331040348357No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13580.14119741228188742No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12880.1339191951539551No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12840.13350329703235897No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12680.13183970454597443No Hit
CCTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAATTTAAATAACTGCTA12580.1307999592419841No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12410.12903239222520052No Hit
ACTTACTACTGTCAACAGAATTACTTTATCCCCCGGACGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAA12360.12851251957320534No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC12350.1284085450428063No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTCGGTCC12320.12809662145160922No Hit
GATTTCACTCTCAGCATCAGCAGTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAATTACTTTATC12110.1259131563132295No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12080.1256012327220324No Hit
AAGTAATTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGGAGACTGCTGATGCTGAGAGTGAAATCTGTC12040.12518533460043627No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11910.12383366570524884No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11760.12227404774926334No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11720.12185814962766721No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11670.12133827697567204No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11600.12061045526287882No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11380.11832301559410008No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11320.11769916841170586No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10970.1140600598477397No Hit
GCCAAGGGACCGAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC10960.11395608531734067No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGGAGACTGCTGATGCTGAGAGTGAAATCTGTCCCAGATCCACTGCCA10840.11270839095255226No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10500.10917325691898513No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA10490.10906928238858608No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10450.10865338426698995No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10360.10771761349339865No Hit
GCGTAGATCAGGACCTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAATT10310.10719774084140347No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10260.10667786818940833No Hit
ACTTTATCCCCCGGACGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGA10090.10491030117262476No Hit
GTCCTGATCTACGCTGCATCCACCTTGCAAAGTGGGGTCCCATCAAGGTT9900.10293478509504311No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9860.10251888697344698No Hit
GATCAGGACCTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAATTTAAAT9830.10220696338224988No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9790.10179106526065375No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT9700.10085529448706244No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9680.10064734542626438No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC9660.10043939636546631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1850.042.9319240
TCGCTAA2000.040.9971831
GTATCAA20950.040.4536931
CGCTAAT2050.040.03278732
CGTATGC2000.039.76870341
GTGGTAT2700.037.8181571
GTATGCC2200.037.77517342
ACTCGCT2350.037.7436329
ATCTCGT2100.037.6322737
AATCTCG2100.037.5967336
GAATACG558.14992E-637.1054579
GCTAATC2250.034.97832533
GCCGTCT2400.034.8244746
CACTCGC2450.034.79794728
CTTATAC2400.034.0363431
CGAGACA2500.034.0310323
ATGCCGT2450.034.01779644
TATCAAC25100.033.469552
CTCGCTA2550.033.4594830
ATCAACG25400.033.2081453
Produced by FastQC (version 0.11.5)