FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961774
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29210.3037096032955767No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25040.26035222411917974No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23880.24829117859289188No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22800.23706192930979625No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20960.2179306157163741No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20160.20961265328445144No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18060.1877780019006544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16690.17353349123598683No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16640.17301361858399167No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16350.16999835720241968No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15940.1657354014560593No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15720.1634479617872806No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15500.16116052211850185No Hit
GATCTACGCTGCATCCACCTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA15000.15596179559855017No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14960.15554589747695405No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT14540.15117896720019464No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14420.14993127283540625No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14230.1479557567578246No Hit
GAATTACTTTATCCCCCGGACGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAACTGTGGCTGCACCAT13920.14473254631545457No Hit
GTCCTGATCTACGCTGCATCCACCTTGCAAAGTGGGGTCCCATCAAGGTT13870.1442126736634594No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGTTATTTAAATTGGTATCAGCAGAAA13810.1435888264810652No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13800.14348485195066618No Hit
ACTTACTACTGTCAACAGAATTACTTTATCCCCCGGACGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAA12850.13360727156275798No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12660.13163175548517636No Hit
GTCTCTGGTGGCTCCATCAGCGATAATAATTGGTGGACTTGGGTCCGCCA12560.13059201018118602No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12540.13038406112038795No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12500.12996816299879183No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12420.12913636675559956No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC12310.12799264692121018No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTCGGTCCCTTGGCCGAACGTC12290.1277846978604121No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11930.12404161476604691No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11860.12331379305325367No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11860.12331379305325367No Hit
CCTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAATTTAAATAACTGCTA11510.11967468448928752No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11450.1190508373068933No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11260.11707532122931168No Hit
GATTTCACTCTCAGCATCAGCAGTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAATTACTTTATC11130.11572365233412424No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11110.11551570327332616No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10840.11270839095255226No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10760.11187659470936No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTCGGTCC10620.11042095128377352No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10580.11000505316217739No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10570.10990107863177835No Hit
GCCAAGGGACCGAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC10320.10730171537180253No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA10250.1065738936590093No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10180.10584607194621606No Hit
AAGTAATTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGGAGACTGCTGATGCTGAGAGTGAAATCTGTC10050.10449440305102863No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9970.10366260680783636No Hit
ACTTTATCCCCCGGACGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGA9920.10314273415584119No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGGAGACTGCTGATGCTGAGAGTGAAATCTGTCCCAGATCCACTGCCA9800.10189503979105279No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)