FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754665
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC23000.3047709911020121No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20410.27045112732139426No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20200.26766843566350634No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG20130.2667408717775437No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT19850.2630306162336931No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG17640.2337460992625867No Hit
TCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTTCTGCAGTTTACATGACGGCTACCACAATGTCTTCGGAA17530.23228849887035968No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17090.2264580973014516No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATT17030.22566304254205508No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16790.22248282350446888No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16330.21638740368242865No Hit
CTCCTGCACTGGATCCAGCAGTGACGTTGGTAGTTATAACTATGTCTCCTGGTATCAGCAACACCCAGACAAAGC14590.19333081565992857No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13410.1776947387251297No Hit
CTATATACACTGGGCGCGACAGGCCCCTGGACAAGGGTTTGAGTGGATGGGATGGATCTACCCTAACAATGGTGG13240.17544208357350613No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13210.17504455619380785No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGATCCAGCAGTGACGTT13040.17279190104218428No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTGGTAGCCGTCATGTAAACTGCAGAAATAATCAGC12610.1670940085998423No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12520.1659014264607475No Hit
GTAGTACACGGCCGTGTCGTCAGATGTCAGCCCGGTCAGTTCCATGTAGGCTGTGTTGATGGACGTGTCGCTGGT12490.1655038990810492No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12470.16523888082791702No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC11680.15477065982919574No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11430.15145793166504343No Hit
GTCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11210.1485427308805894No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTGGTAGC11080.1468201122352302No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10670.1413872380460204No Hit
GTCAGTCACCATCTCCTGCACTGGATCCAGCAGTGACGTTGGTAGTTATAACTATGTCTCCTGGTATCAGCAACA10510.13926709202096293No Hit
GGATCCAGCAGTGACGTTGGTAGTTATAACTATGTCTCCTGGTATCAGCA10510.13926709202096293No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT10090.13370170870518708No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9380.12429356071899451No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9380.12429356071899451No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9190.12177588731423876No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8950.11859566827665255No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT8920.11819814089695428No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8860.11740308613755772No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA8760.1160779948718968No Hit
GACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTGGTAGCCGTCATGTAAACTGCAGAAATAATCAG8750.1159454857453307No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT8720.11554795836563243No Hit
GTGTACTACTGTGCGAGAGATTCAATCAAAAATGCTTTTGATATCTGGGG8660.11475290360623588No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG8470.11223523020148013No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8460.11210272107491402No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8360.11077762980925311No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT8250.1093200294170261No Hit
GGATGAGGCTGATTATTTCTGCAGTTTACATGACGGCTACCACAATGTCTTCGGAACTGGGACCAAGGTCACCGT8150.10799493815136518No Hit
CACCCAGACAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCT8080.10706737426540254No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7840.10388715522781632No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC7780.10309210046841977No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7720.1022970457090232No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC7700.10203202745589102No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT7670.10163450007619275No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA7640.10123697269649448No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT7600.1007069361902301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)