FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754665
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23180.3071561553802018No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC22830.3025183359503886No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG19600.2597178880695408No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18830.24951468532395168No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18740.24832210318485687No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18700.2477920666785925No Hit
TCTCTGGACTCCAGGCTGAGGATGAGGCTGATTATTTCTGCAGTTTACATGACGGCTACCACAATGTCTTCGGAA17560.232686026250058No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC16890.2238079147701298No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16260.21545983979646596No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG15780.20909940172129357No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATT14980.19849867159600618No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC14610.19359583391306076No Hit
CTCCTGCACTGGATCCAGCAGTGACGTTGGTAGTTATAACTATGTCTCCTGGTATCAGCAACACCCAGACAAAGC14040.1860428136987935No Hit
CTATATACACTGGGCGCGACAGGCCCCTGGACAAGGGTTTGAGTGGATGGGATGGATCTACCCTAACAATGGTGG14010.18564528631909522No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13750.18220004902837683No Hit
GTAGTACACGGCCGTGTCGTCAGATGTCAGCCCGGTCAGTTCCATGTAGGCTGTGTTGATGGACGTGTCGCTGGT12710.16841909986550324No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC12310.16311873480285954No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12310.16311873480285954No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12290.16285371654972736No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12080.16007102489183944No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGATCCAGCAGTGACGTT12070.15993851576527335No Hit
GGATCCAGCAGTGACGTTGGTAGTTATAACTATGTCTCCTGGTATCAGCA11100.1470851304883624No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11090.1469526213617963No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTGGTAGCCGTCATGTAAACTGCAGAAATAATCAGC11070.1466876031086641No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10720.14204978367885088No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTGGTAGC10310.13661690948964111No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10070.13343669045205486No Hit
GTCAGTCACCATCTCCTGCACTGGATCCAGCAGTGACGTTGGTAGTTATAACTATGTCTCCTGGTATCAGCAACA9940.1317140718066957No Hit
GTGTACTACTGTGCGAGAGATTCAATCAAAAATGCTTTTGATATCTGGGG9910.13131654442699742No Hit
GTCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG9860.13065399879416695No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9170.12151086906110659No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG9020.1195232321626152No Hit
GTGTATATAGTAGCCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCCTCACTGAGGCCCCAGGCTTCTTCAC8860.11740308613755772No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC8720.11554795836563243No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT8650.11462039447966979No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG8310.11011508417642264No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8270.10958504767015827No Hit
GACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTGGTAGCCGTCATGTAAACTGCAGAAATAATCAG8170.10825995640449736No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7920.10494722824034505No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA7790.10322460959498586No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT7590.10057442706366401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)