FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715014
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27140.37957298738206524No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22760.3183154455716951No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC19960.27915537318150413No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17830.24936574668468028No Hit
GTAATACACGGCAGTGTCCTCAGCCTTTAGACTACTGATCTGCAGATATG17610.24628888385402245No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATT16540.23132414190491377No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15490.21663911475859216No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15080.21090496130145706No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15010.20992595949170229No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14720.20587009485128963No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGG14570.20377223383038653No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14560.203632376428993No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTCCTTGGCCTGGTACC13460.18824806227570368No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACACCGCCACTGGGAAGGCAACGTATGCGCACGACTTCA13400.18740891786734246No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGAGGCCAGAGGCCCTGTT13130.1836327680297169No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGC12950.1811153348046332No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12820.17929718858651716No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12650.17691961276282703No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12630.17663989796003995No Hit
CTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTTTTTTACTGTCAAC12500.17482175174192394No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12460.1742623221363498No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA12140.16978688529175653No Hit
GTGCAGCCACAGTTCGTTTCCTCTCCAGCTTGGTCCCCTGGCCAAAAGTG11950.16712959466527927No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11820.16531144844716328No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGC11460.16027658199699585No Hit
GACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTTTT11390.15929758018724108No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11280.15775914877191216No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11110.155381572948222No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGAGGCCA10880.1521648527161706No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10850.15174528051198996No Hit
GTGTATTACTGTGCGAGAGATCGGGGACGAAACTTTGACTATTGGGGCCA10760.15048656389944812No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10560.14768941587157736No Hit
GGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTCCTTGGCCTGGTACCAGCACAAAC10320.14433283823813242No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10220.142934264224197No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG10150.14195526241444223No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10100.14125597540747453No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCCTCTCCAGCTTGGTCC10040.14041683099911328No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9870.13803925517542312No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9720.13594139415452006No Hit
GTCAGAGTGTTAGCGGCAACTCCTTGGCCTGGTACCAGCACAAACCTGGC9490.13272467392246864No Hit
GCACAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCTCTGGCCTCCCAGACAGGTT9460.13230510171828802No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT9280.12978766849320433No Hit
TCTATGATGCATCCAACAGGGCCTCTGGCCTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9150.12796952227508832No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9080.12699052046533355No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8930.12489265944443045No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8920.12475280204303692No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8900.12447308724024984No Hit
CTGCTACTCTGGCTCCCAGATGCCAGCGGAGAAATTGTGTTGACGCAGTC8720.12195565401516614No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8600.12027736519844366No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8550.11957807819147598No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8500.11887879118450827No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8500.11887879118450827No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCAGCGGAGAAATTGTGTTGACGCAGTCTC8450.11817950417754058No Hit
GTCCAGGGGCCTGTCGCACCCAACTTATAGAATAGGCAGTGAAGCTGTAACCAGAAGCCTTGCAGGAAACCTTCA8290.11594178575524394No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8230.11510264134688272No Hit
AACCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCC8130.11370406733294733No Hit
CTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCTC8090.11314463772737317No Hit
GTACCAGCACAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCTCTGGCCTCCCAGA8040.11244535072040547No Hit
CATCTATGATGCATCCAACAGGGCCTCTGGCCTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA8030.11230549331901195No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7950.11118663410786363No Hit
CTCCTTGGCCTGGTACCAGCACAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCT7930.11090691930507654No Hit
CAGTAATACACGGCAGTGTCCTCAGCCTTTAGACTACTGATCTGCAGATA7900.11048734710089592No Hit
GCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGT7880.11020763229810884No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7840.1096482026925347No Hit
GTCTGGGAGGCCAGAGGCCCTGTTGGATGCATCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTGTGCTGGTA7680.10741048427023807No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7650.10699091206605743No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7590.10615176765769621No Hit
GGTTTGTGCTGGTACCAGGCCAAGGAGTTGCCGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTATCC7540.10545248065072853No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7520.10517276584794144No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7500.10489305104515437No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGAGGAAACGAACTGTGGCTGCACCA7360.10293504742564481No Hit
GTATTACTGTGCGAGAGATCGGGGACGAAACTTTGACTATTGGGGCCAGG7300.10209590301728357No Hit
GGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCTCCATCAGCAGA7260.10153647341170943No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7200.10069732900334818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.048.811711
AGGCTAG306.860195E-445.2258575
TGGTATC1350.045.2195362
GTGGTAT1650.041.120171
ATGCCGT1350.038.60625544
TCGTATG1350.038.3152840
ATCTCGT1350.038.09317837
CGTCTTC1400.037.53943648
AGACGTA1450.037.47022625
GCCGTCT1400.037.3881846
GTATCAA17150.035.803321
AATCTCG1450.035.40349636
ACGTAGA1550.035.0724427
GACGTAG1550.035.0650726
CACGAGA1600.033.93363621
CCCGATG400.002818982833.924147
TATACAC1600.033.9146543
TATAACT400.00282285233.9146544
GTATGCC1650.033.55570242
GAATCTC1550.033.0867135
Produced by FastQC (version 0.11.5)