FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715014
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27430.3836288520224779No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20180.282232236012162No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19910.27845608617453643No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC18660.26097391100034406No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17070.2387365841787713No Hit
GTAATACACGGCAGTGTCCTCAGCCTTTAGACTACTGATCTGCAGATATG16180.22628927545474634No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATT16140.22572984584917222No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15920.22265298301851433No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15270.2135622519279343No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14640.2047512356401413No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTCCTTGGCCTGGTACC13590.19006620849381972No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13520.18908720668406492No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGG13430.18782849007152308No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACACCGCCACTGGGAAGGCAACGTATGCGCACGACTTCA13070.18279362362135565No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGAGGCCAGAGGCCCTGTT12340.1725840333196273No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12250.17132531670708545No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGC12220.17090574450290485No Hit
GACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTTTT12180.17034631489733068No Hit
CTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTTTTTTACTGTCAAC12120.16950717048896943No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12100.16922745568618236No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11870.16601073545413095No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11740.16419258923601496No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11680.16335344482765374No Hit
GTGCAGCCACAGTTCGTTTCCTCTCCAGCTTGGTCCCCTGGCCAAAAGTG11320.1583185783774863No Hit
GTGTATTACTGTGCGAGAGATCGGGGACGAAACTTTGACTATTGGGGCCA11170.15622071735658322No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10790.15090613610362874No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10630.14866841768133213No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGAGGCCA10570.14782927327297088No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10560.14768941587157736No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10270.1436335512311647No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10160.14209511981583578No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGC10110.14139583280886808No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10090.141116118006081No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9870.13803925517542312No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9780.13678053856288128No Hit
GGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTCCTTGGCCTGGTACCAGCACAAAC9610.13440296273919114No Hit
GCACAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCTCTGGCCTCCCAGACAGGTT9570.13384353313361697No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCCTCTCCAGCTTGGTCC9360.13090652770435265No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG9310.13020724069738493No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9020.12615137605697233No Hit
TCTATGATGCATCCAACAGGGCCTCTGGCCTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9010.12601151865557877No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9000.12587166125418522No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8980.12559194645139815No Hit
CTGCTACTCTGGCTCCCAGATGCCAGCGGAGAAATTGTGTTGACGCAGTC8930.12489265944443045No Hit
GTCAGAGTGTTAGCGGCAACTCCTTGGCCTGGTACCAGCACAAACCTGGC8580.11999765039565659No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8580.11999765039565659No Hit
GTCCAGGGGCCTGTCGCACCCAACTTATAGAATAGGCAGTGAAGCTGTAACCAGAAGCCTTGCAGGAAACCTTCA8560.11971793559286951No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8450.11817950417754058No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCAGCGGAGAAATTGTGTTGACGCAGTCTC8190.11454321174130856No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8100.11328449512876672No Hit
CTCCTTGGCCTGGTACCAGCACAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCT8080.11300478032597963No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8060.11272506552319256No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8000.11188592111483132No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7840.1096482026925347No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7720.10796991387581222No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7710.10783005647441869No Hit
CTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCTC7630.10671119726327037No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7590.10615176765769621No Hit
CATCTATGATGCATCCAACAGGGCCTCTGGCCTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA7550.10559233805212205No Hit
GCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGT7540.10545248065072853No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7480.10461333624236728No Hit
GTATTACTGTGCGAGAGATCGGGGACGAAACTTTGACTATTGGGGCCAGG7390.10335461962982544No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7320.10237561782007065No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7280.1018161882144965No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7220.10097704380613527No Hit
CAGTAATACACGGCAGTGTCCTCAGCCTTTAGACTACTGATCTGCAGATA7210.10083718640474172No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7190.10055747160195466No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACT200.00793290550.77433416
GTGGTAT1600.046.559431
TAGGACA452.1301166E-645.1043364
GGTATCA6100.041.6328741
GTATCAA16300.038.639391
TGGTATC1850.038.4023252
ACGTACC754.7615686E-836.0960913
TACGTAC754.7702997E-836.08851212
CGTACCA906.2555046E-933.8424514
GAATACG808.414827E-833.8329859
TATCAAC18650.033.195732
ATCAACG19200.032.2425543
TCAACGC19150.032.150094
AATACGT851.4329999E-731.8428110
AGGTCGC550.0038716631.79519769
CAACGCA19650.031.3364015
AACGCAG19900.031.1127456
ATACGTA902.3653774E-730.07376311
AACCTTC3700.029.5342168
ACGCAGA20900.029.4622157
Produced by FastQC (version 0.11.5)