FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481280
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCGTCGCCTCTCAGAAGATTCATTTTAAGATATA75950.5127322315834953No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53590.36178170231151435No Hit
GTATATTACTGTGCAGGCTCGGGGGGAATTCCGCAGTGGTTAATCTGGGGCAAAGGGACCACAGTCACCGTCTC52430.353950637286671No Hit
GTTTAGGAGGTTGGCCTGGTTTCTGGAGGTACCAACCTATGTAGTTCTTA51740.34929250378051413No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50960.34402678764311945No Hit
CTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT43090.2908970620004321No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAATCTTTTGTT37300.25180924605746385No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34220.23101641823287966No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33000.22278029812054437No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29390.1984094836897818No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28620.19321127673363578No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28600.19307625837113848No Hit
CTACATAGGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATC28280.19091596457118168No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27680.1868654136962627No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27350.18463761071505724No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27280.1841650464463167No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27110.18301739036508965No Hit
GTTTATTACTGTCAGCAATATTTTAGTACTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA27010.18234229855260314No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT26050.17586141715273276No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25730.173701123352776No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTTC24840.16769280622164615No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24830.1676252970403975No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24500.16539749405919205No Hit
CTCCTGTGCAGCCTCTCAATTCTCCTTCAGCAACTACTGGATACACTGGGTCCGCCAAGTTCCAGGGAAGGGG24240.16364225534672716No Hit
CCTATGTAGTTCTTACTGTTGGAGCCGGAGTTGAACAAAAGATTCTGGCTGGACTTGCAATTGATGGTGGCCCT23980.16188701663426225No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG23560.15905163102181896No Hit
GAACTACATAGGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGC23460.15837653920933245No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22630.15277327716569453No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22410.15128807517822424No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT21750.14683246921581336No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21310.14386206524087275No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21290.14372704687837548No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20740.1400140419096997No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20590.13900140419096996No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20450.13805627565348888No Hit
CCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGAT20430.13792125729099158No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20200.13636854612227264No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20200.13636854612227264No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19910.13441077986606179No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19910.13441077986606179No Hit
GGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTC19710.1330605962410888No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19590.132250486066105No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAATCTTTTGTTCAACTCCGG19270.13009019226614818No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC18960.12799740764744005No Hit
GTCCCTGACCGATTCAGTGGCAGCGGATCTGGGACAGATTTCACTCTCAC18910.1276598617411968No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18890.12752484337869952No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTAGTACT18800.12691726074746165No Hit
ATGTAGTTCTTACTGTTGGAGCCGGAGTTGAACAAAAGATTCTGGCTGGACTTGCAATTGATGGTGGCCCTCTCG18760.12664722402246706No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18470.1246894577662562No Hit
GCCCTGGGATTCCCACCTGTTTCTGCTTGCTGATCAGGACTGCACACAGA18380.12408187513501835No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18360.12394685677252107No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCA18300.12354180168502917No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17910.12090894361633181No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17800.12016634262259668No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17640.11908619572261828No Hit
ATTTTAGTACTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAA17560.11854612227262908No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17480.11800604882263988No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17390.11739846619140203No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17240.11638582847267229No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC16980.11463058976020739No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16940.1143605530352128No Hit
CCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG16880.11395549794772089No Hit
ACATAGGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGCTCATT16640.11233527759775329No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16500.11139014906027218No Hit
GGTTAATCTGGGGCAAAGGGACCACAGTCACCGTCTCCTCAGCATCCCCG16470.11118762151652625No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAATCTTTTGTTCAACTCCGGCT16430.11091758479153166No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16400.11071505724778569No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16240.1096349103478073No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16170.10916234607906675No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAGTTTAGGAGGTTGGCCTGGTTTCTGGAGGTA15530.10484175847915317No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15410.10403164830416936No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15360.10369410239792613No Hit
GTACTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT14990.10119626269172606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4000.052.6581541
TCGTATG4050.051.9296140
ATCTCGT4100.048.54888537
ACATGCG4300.048.26031527
ACGAGAC4400.047.8621922
TATACAC4700.046.9546363
CACGAGA4550.046.28275721
ATGCCGT4700.045.77326644
CTTATAC4650.045.2996671
ATCTCCG4750.045.0384310
GTATGCC4900.043.76154742
GGTATCA12200.043.7216071
TTATACA4900.043.6523322
CGCAGAT4650.043.3096332
CGAGACA5000.042.80529423
TCTCCGA5000.042.78650711
TACACAT5250.042.0384185
TGCTTGA5400.042.0177655
CCCACGA5050.041.69326819
GCCGTCT5200.041.50802646
Produced by FastQC (version 0.11.5)