FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481280
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCGTCGCCTCTCAGAAGATTCATTTTAAGATATA68370.46156027219701873No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51340.34659213653056814No Hit
GTATATTACTGTGCAGGCTCGGGGGGAATTCCGCAGTGGTTAATCTGGGGCAAAGGGACCACAGTCACCGTCTC50040.3378159429682437No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50030.33774843378699504No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46740.31553791315618923No Hit
GTTTAGGAGGTTGGCCTGGTTTCTGGAGGTACCAACCTATGTAGTTCTTA46450.3135801468999784No Hit
CTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT35940.24262799740764743No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34420.23236660185785266No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAATCTTTTGTT32280.2179196370706416No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31060.20968351695830634No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27490.18558273925253835No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27380.1848401382588032No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27060.1826798444588464No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26920.1817347159213653No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26870.18139717001512207No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCA26400.1782242384964355No Hit
GTTTATTACTGTCAGCAATATTTTAGTACTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA25770.17397116007777058No Hit
CTCCTGTGCAGCCTCTCAATTCTCCTTCAGCAACTACTGGATACACTGGGTCCGCCAAGTTCCAGGGAAGGGG25690.17343108662778137No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25410.17154082955281919No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24640.16634262259667315No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTTC23970.16181950745301363No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22670.15304331389068912No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG22540.1521656945344567No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22130.14939781810326205No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21870.14764257939079714No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21550.14548228559084037No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21420.1446046662346079No Hit
CCTATGTAGTTCTTACTGTTGGAGCCGGAGTTGAACAAAAGATTCTGGCTGGACTTGCAATTGATGGTGGCCCT21340.1440645927846187No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21330.14399708360337007No Hit
CTACATAGGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATC21230.14332199179088356No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20990.14170177144091597No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20790.14035158781594295No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20350.1373811838410024No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC19940.13461330740980773No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19860.13407323395981854No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19820.13380319723482395No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19690.1329255778785915No Hit
GAACTACATAGGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGC19280.13015770144739683No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19020.12840246273493194No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18900.12759235255994814No Hit
GAGTGAAATCTGTCCCAGATCCGCTGCCACTGAATCGGTCAGGGACCCCG18410.12428440267876431No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17920.12097645279758047No Hit
GTACTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT17740.11976128753510477No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17270.11658835601641823No Hit
GTCCCTGACCGATTCAGTGGCAGCGGATCTGGGACAGATTTCACTCTCAC17230.11631831929142364No Hit
ATGTAGTTCTTACTGTTGGAGCCGGAGTTGAACAAAAGATTCTGGCTGGACTTGCAATTGATGGTGGCCCTCTCG17220.11625081011017499No Hit
CCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGAT17150.11577824584143445No Hit
GCCCTGGGATTCCCACCTGTTTCTGCTTGCTGATCAGGACTGCACACAGA17110.11550820911643983No Hit
CCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG17110.11550820911643983No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17100.1154406999351912No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC17060.11517066321019657No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAATCTTTTGTTCAACTCCGG16770.11321289695398574No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16500.11139014906027218No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16480.1112551306977749No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTAGTACT16330.11024249297904515No Hit
GGTTGGTACCTCCAGAAACCAGGCCAACCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTC16050.10835223590408295No Hit
ATTTTAGTACTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAA15800.1066645063728667No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15720.10612443292287752No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15690.10592190537913157No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15670.10578688701663426No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15500.10463923093540722No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15400.10396413912292073No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15290.10322153812918557No Hit
GGTTAATCTGGGGCAAAGGGACCACAGTCACCGTCTCCTCAGCATCCCCG15270.10308651976668826No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA15180.10247893713545041No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAGTTTAGGAGGTTGGCCTGGTTTCTGGAGGTA15100.10193886368546122No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCACTGAATCGGTCAGGGACC15000.10126377187297472No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14980.10112875351047743No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14970.10106124432922878No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14870.10038615251674228No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14870.10038615251674228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT250.00699497352.41831669
GGTATCA11500.038.6399081
GTATCAA29250.038.153351
CGTTGTC15950.035.00619563
TTAAGAT15900.034.77448741
GCGTTGT16100.034.77207662
TAAGATA15950.034.51342
GGCGTTG16500.034.02310661
TATCAAC32850.033.930872
GTTGTCT16500.033.71581664
TATACCG16350.033.51338247
TTTAAGA16450.033.353540
TACCGTG16200.033.30479449
ATACCGT16350.032.9371548
GATATAC16900.032.9197545
CATTTTA16650.032.57534437
AGATATA17300.032.5166444
TTGGCGT17000.032.4096259
TGGCGTT16950.032.39694660
ATCAACG34400.032.396543
Produced by FastQC (version 0.11.5)